DNA-based stable isotope probing identifies formate-metabolizing methanogenic archaea in paddy soil
DNA-based stable isotope probing identifies formate-metabolizing methanogenic archaea in paddy soil
复制标题
基于DNA的稳定同位素探测鉴定水稻土中的甲酸代谢产甲烷古菌
DOI：
10.1016/j.micres.2017.05.004
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发表时间：
2017
影响因子：
6.7
通讯作者：
Feng Youzhi
中科院分区：
文献类型：
--
作者：
Xu Jiangbing;Jia Zhongjun;Lin Xiangui;Feng Youzhi
稻田甲烷（CH 4）的产生是由代谢各种有机和/或无机碳源的产甲烷古菌生物主导的。虽然甲酸很容易被异化为H2/CO2，但代谢甲酸的产甲烷古菌与利用CO2的产甲烷菌不同。水稻土中甲酸盐代谢产甲烷古菌的身份仍然难以捉摸。本研究利用稳定同位素探针（SIP）技术对水稻土中代谢甲酸根的产甲烷古菌进行了分子鉴定。CH 4排放监测、实时定量PCR（qPCR）和变性梯度凝胶电泳（DGGE）分析一致表明，微宇宙中部分产甲烷古菌代谢13 C标记的甲酸盐，占缺氧稻田土壤中甲酸盐代谢古菌的很大一部分。系统发育鉴定进一步发现该类群隶属于甲烷杆菌科。综合来看，甲烷细菌可能是稻田土壤中优势的甲酸盐代谢产甲烷古菌，在甲烷的产生中起着重要的作用。这些发现将扩展现有的知识，水稻产甲烷古菌和微生物驱动的水稻CH 4排放。
Paddy methane (CH4) production is biologically dominated by methanogenic archaea that metabolize a variety of organic and/or inorganic carbon sources. Though formate is easily dissimilated into H2/CO2, formate-metabolizing methanogenic archaea are distinct from CO2-utilizing methanogen taxa. The identity of formate-metabolizing methanogenic archaea in paddy soil remains elusive. In this investigation, molecular approaches based on stable isotope probing (SIP) technique were conducted to identify the formate-metabolizing methanogenic archaea in paddy soil. CH4emission monitor, real-time quantitative PCR (qPCR) and Denaturing Gradient Gel Electrophoresis (DGGE) analyses consistently indicated that some methanogenic archaea metabolized13C-labeled formate in microcosm and accounted for a large portion of formate-metabolizing archaea in anoxic paddy soil. Phylogenetic identification further found that this guild was affiliated toMethanobacteriaceae. Taken togetherMethanobacteriaceaecould be the dominant formate-metabolizing methanogenic archaea and play an important role in the CH4production in paddy soil. These findings would extend the extant knowledge on paddy methanogenic archaea and microbial-driven paddy CH4emission.