PROGNOSTIC-SIGNIFICANCE OF CYTOPLASMIC-P53 ONCOPROTEIN IN COLORECTAL ADENOCARCINOMA
PROGNOSTIC-SIGNIFICANCE OF CYTOPLASMIC-P53 ONCOPROTEIN IN COLORECTAL ADENOCARCINOMA
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DOI：
10.1016/0140-6736(92)92558-w
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发表时间：
1992-12-05
期刊：
影响因子：
168.9
通讯作者：
NORDENSKJOLD, B
中科院分区：
文献类型：
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作者：
SUN, XF;CARSTENSEN, JM;NORDENSKJOLD, B
肿瘤抑制蛋白p53的突变导致蛋白质的过度表达和其肿瘤抑制特性的丧失。在某些肿瘤（如乳腺癌）中，p53表达与众所周知的预后因素有关，但在结直肠肿瘤中的发现是模棱两可的。我们用多克隆抗体CM 1研究了p53在结直肠肿瘤细胞核和细胞质中的表达，并评估了它们与预后的关系。在293例结直肠腺癌中，71例（24%）显示p53仅在细胞核中表达，30例（10%）显示p53仅在细胞质中表达，43例（15%）显示细胞核和细胞质均表达。核p53表达与肿瘤的生存期或Dukes分期无关。然而，细胞质表达频率随着Dukes分期的进展而增加（趋势卡方系数为11.18，1 df，p=0.0008），细胞质表达与低生存率相关（率比为2.3 [95%CI 1.6-3.3]，p
Mutation of p53, a tumour-suppressor protein, leads to overexpression of the protein and loss of its tumour-suppressive properties. In some tumours (eg, breast) p53 expression is related to well-known prognostic factors, but findings in colorectal tumours are equivocal. We have used the polyclonal antibody CM1 to investigate nuclear and cytoplasmic p53 expression in colorectal tumours and to assess their relations with prognosis.Of 293 colorectal adenocarcinomas, 71 (24%) showed p53 expression in the nucleus alone, 30 (10%) showed p53 in the cytoplasm alone, and 43 (15%) showed both nuclear and cytoplasmic expression. Nuclear p53 expression showed no relation with survival or Dukes' stage of the tumour. However, the frequency of cytoplasmic expression increased with advancing Dukes' stage (chi2 for trend 11.18, 1 df, p=0.0008) and cytoplasmic expression was associated with poor survival (rate ratio 2.3 [95% Cl 1.6-3.3], p