Control of Lipid Bilayer Phases of Cell-Sized Liposomes by Surface-Engineered Plasmonic Nanoparticles
Control of Lipid Bilayer Phases of Cell-Sized Liposomes by Surface-Engineered Plasmonic Nanoparticles
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通过表面工程等离子体纳米颗粒控制细胞大小的脂质体的脂质双层相
DOI：
10.1021/acs.langmuir.0c00049
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发表时间：
2020
期刊：
影响因子：
3.9
通讯作者：
Tatsuya Murakami
中科院分区：
文献类型：
--
作者：
Tomohiro Nobeyama;Kazuki Shigyou;Hirotaka Nakatsuji;Hiroshi Sugiyama;Naoko Komura;Hiromune Ando;Tsutomu Hamada;Tatsuya Murakami
液序（Lo）相域是脂双层上富含胆固醇的区域，最近因其与脂筏的相关性而引起了极大的关注，脂筏的形成/崩溃与通过质膜的各种信息交换有关。在这里，我们证明了细胞大小的脂质体（即巨型单层囊泡（GUV））中 Lo 相域的形成/崩溃可以通过生物活性等离子体纳米粒子和光来控制。该纳米颗粒是通过使用高密度脂蛋白（HDL）（一种天然胆固醇转运蛋白）的阳离子化突变体对金纳米棒（AuNR）进行表面修饰来制备的。将表面工程化的 AuNR 添加到具有 Lo 和液相无序 (Ld) 相混合域的 GUV 中后，Lo 域塌陷并形成固序 (So) 相域。 AuNRs 负载胆固醇，通过光热实现反向相变。在这些转变过程中，AuNR 似乎选择性地定位在相混合 GUV 中的流体较少的区域（Lo 或 So）上。这些结果表明，相变是通过 AuNR 的膜结合发生的，然后是 AuNR 和 GUV 之间胆固醇的自发/光热转移。我们开发生物活性 AuNR 的策略可能能够时空控制活细胞中脂筏的形成/崩溃。
Liquid-ordered (Lo)-phase domains, a cholesterol-rich area on lipid bilayers, have attracted significant attention recently because of their relevance to lipid rafts, the formation/collapse of which is associated with various kinds of information exchange through the plasma membrane. Here, we demonstrate that the formation/collapse of Lo-phase domains in cell-sized liposomes, that is, giant unilamellar vesicles (GUVs), can be controlled with bioactive plasmonic nanoparticles and light. The nanoparticles were prepared by surface modification of gold nanorods (AuNRs) using a cationized mutant of high-density lipoprotein (HDL), which is a natural cholesterol transporter. Upon the addition of surface-engineered AuNRs to GUVs with the mixed domains of Lo and liquid-disorder (Ld) phases, the Lo domains collapsed and solid-ordered (So)-phase domains were formed. The reverse phase transition was achieved photothermally, with the AuNRs loaded with cholesterol. During these transitions, the AuNRs appeared to be selectively localized on the less fluidic domain (Lo or So) in the phase-mixed GUVs. These results indicate that the phase transitions occur through the membrane binding of the AuNRs followed by spontaneous/photothermal transfer of cholesterol between the AuNRs and GUVs. Our strategy to develop bioactive AuNRs potentially enables spatiotemporal control of the formation/collapse of lipid rafts in living cells.