Yield quantitative trait loci from wild tomato are predominately expressed by the shoot
Yield quantitative trait loci from wild tomato are predominately expressed by the shoot
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DOI：
10.1007/s00122-010-1456-9
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Zamir, Dani
中科院分区：
文献类型：
--
作者：
Gur, Amit;Semel, Yaniv;Zamir, Dani
植物产量是地上和地下过程的综合结果。为了探索番茄果实产量的遗传、环境和发育方面，我们表型分析了栽培番茄（番茄）和野生种（潘那利番茄）杂交的渐渗系（IL）群体。纯合和杂合IL在灌溉和非灌溉的领域，并评估了六个产量组成部分。13个品系表现出超亲分离，在2年内持续增加农艺产量，并确定了至少11个独立的增产QTL。为了确定这些QTL是否在植物的芽或根中表达，我们进行了嫁接IL的田间试验;在对产量有影响的13个品系中，10个QTL在芽中有活性，并且仅IL 8 -3显示出一致的根效应。为了进一步研究这种不寻常的情况下，我们评估了来自IL 8 -3的纯合和杂合系的果实的代谢谱，并将这些代谢谱与从根效应群体中其等同基因型的果实获得的代谢谱进行比较。我们观察到这些代谢QTL中的几个，如产量QTL，是根决定的，然而，需要进一步的研究来描绘在这个特定的线介导这种效果的确切机制。这里提出的结果表明，根性状的遗传变异，相比，目前在地上部，代表番茄果实产量的决定只有一个次要组成部分。
Plant yield is the integrated outcome of processes taking place above and below ground. To explore genetic, environmental and developmental aspects of fruit yield in tomato, we phenotyped an introgression line (IL) population derived from a cross between the cultivated tomato (Solanum lycopersicum) and a wild species (Solanum pennellii). Both homozygous and heterozygous ILs were grown in irrigated and non-irrigated fields and evaluated for six yield components. Thirteen lines displayed transgressive segregation that increased agronomic yield consistently over 2 years and defined at least 11 independent yield-improving QTL. To determine if these QTL were expressed in the shoots or the roots of the plants, we conducted field trials of reciprocally grafted ILs; out of 13 lines with an effect on yield, 10 QTL were active in the shoot and only IL8-3 showed a consistent root effect. To further examine this unusual case, we evaluated the metabolic profiles of fruits from both the homo- and heterozygous lines for IL8-3 and compared these to those obtained from the fruit of their equivalent genotypes in the root effect population. We observed that several of these metabolic QTL, like the yield QTL, were root determined; however, further studies will be required to delineate the exact mechanism mediating this effect in this specific line. The results presented here suggest that genetic variation for root traits, in comparison to that present in the shoot, represents only a minor component in the determination of tomato fruit yield.