Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI.
Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI.
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DOI：
10.1016/j.xpro.2022.101499
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发表时间：
2022-09-16
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由于检测灵敏度和对比度的限制，129Xe MRI在活细胞中成像基质仍然是一个挑战。在这里，我们提出了一种有效的方案，用超极化129Xe MRI检测和成像人肺癌细胞A549中的底物。该方案针对一种对生物硫醇敏感的隐烷探针进行了优化，并可扩展到其他基于x的探针，以检测其他哺乳动物细胞中的潜在生物标志物。有关使用和执行本协议的完整详情，请参阅。使用超极化129Xe MRI检测活细胞中底物的方案超极化129Xe MRI培养和收集细胞的程序为对生物硫醇敏感的基于加密素的探针优化出版商注：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指导方针。由于检测灵敏度和对比度的限制，129Xe MRI在活细胞中成像基质仍然是一个挑战。在这里，我们提出了一种有效的方案，用超极化129Xe MRI检测和成像人肺癌细胞A549中的底物。该方案针对一种对生物硫醇敏感的隐烷探针进行了优化，并可扩展到其他基于x的探针，以检测其他哺乳动物细胞中的潜在生物标志物。
Due to limited detection sensitivity and contrast limitation, imaging substrates with 129Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized 129Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells. For complete details on the use and execution of this protocol, please refer to. Protocol for detecting substrates in living cells with hyperpolarized 129Xe MRI Procedure for culturing and collecting cells for hyperpolarized 129Xe MRI Optimized for a cryptophane-based probe sensitive to biothiols Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Due to limited detection sensitivity and contrast limitation, imaging substrates with 129Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized 129Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells.