Cryogenic frozen device for hepatocyte culture and responses
Cryogenic frozen device for hepatocyte culture and responses
复制标题
用于肝细胞培养和反应的低温冷冻装置
DOI：
10.1109/nems.2012.6196745
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Cheng
中科院分区：
文献类型：
--
作者：
L. Ke;Yu;Jing Liu;Cheng
在临床医学中，冷冻治疗已应用于患者多年。然而，微/纳米生物芯片（或实验室芯片）技术和低温冷冻技术的结合在过去很少被提出。本文报道了一种用于在三维肝细胞培养芯片上研究微流控肝组织模拟肝索反应的低温冷冻装置。我们设计了高度约为85 μ m的微型柱体，用于加载肝细胞，形成三维组织模拟结构。肝脏被组织成多棱柱形的小叶。每个小叶在横截面上典型地是六边形的，并且以中央静脉为中心。小叶的主体由肝细胞组成，肝细胞排列成肝索，肝索被窦状隙隔开。在肝功能检测中，冷冻肝细胞10分钟，白蛋白分泌受影响12%。
In clinical medicine, freezing treatment has been applied to patients for years. However, the combination of the micro/nano biochip (or Lab Chip) techniques and cryogenic frozen techniques were seldom proposed in the past. This paper reports a cryogenic frozen apparatus for the study of microfluidic liver tissue mimic hepatic cords responses on three dimension hepatocyte culture chip. We designed the micro-cylinder about the height of 85 micrometer for loading hepatocyte to form three dimension tissue-mimic structures. The liver is organized into lobules which take the shape of polygonal prisms. Each lobule is typically hexagonal in cross section and is centered on the central vein. The bulk of the lobule consists of hepatocytes which are arranged into hepatic cords that are separated by sinusoid space. In liver function testing, the albumin secretion was affected 12% by freezing the hepatocyte ten minutes.
DOI：
10.1115/1.3156804
发表时间：
2009-07
期刊：
Journal of biomechanical engineering
影响因子：
--
作者：
Goel R;Anderson K;Slaton J;Schmidlin F;Vercellotti G;Belcher J;Bischof JC
通讯作者：
Bischof JC