The melatonin metabolite N1-acetyl-5-methoxykynuramine is a potent singlet oxygen scavenger
The melatonin metabolite N1-acetyl-5-methoxykynuramine is a potent singlet oxygen scavenger
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DOI：
10.1111/j.1600-079x.2008.00614.x
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发表时间：
2009-01-01
影响因子：
10.3
通讯作者：
Hardeland, Rudiger
中科院分区：
文献类型：
--
作者：
Schaefer, Meike;Hardeland, Rudiger
利用玫瑰红在可见光照射下产生单线态氧。N-1-乙酰基-5-甲氧基犬尿胺（AMK）被这种活性氧物质迅速破坏，而其甲酰化前体N-1-乙酰基-N-2-甲酰基-5-甲氧基犬尿胺（AFMK）是显著惰性的。在光子注量率为1400 μ mol光子/m（2）s时，使用20 μ m玫瑰红，最初0.2 mm的AMK在2分钟内被破坏，而AFMK在更长的时间内几乎保持不变。与其他清除剂的竞争实验揭示了对单线态氧的反应性顺序：二氮杂双环[2，2，2]辛烷（DABCO）
Singlet oxygen was generated by means of rose bengal under irradiation by visible light. N-1-acetyl-5-methoxykynuramine (AMK) was rapidly destroyed by this reactive oxygen species, whereas its formylated precursor, N-1-acetyl-N-2-formyl-5-methoxykynuramine (AFMK), was remarkably inert. At photon fluence rates of 1400 mu mol photons/m(2)s, and using 20 mu m rose bengal, most of initially 0.2 mm AMK was destroyed within 2 min, whereas AFMK remained practically unchanged for much longer periods of time. Competition experiments with other scavengers revealed the following order of reactivity towards singlet oxygen: diazabicyclo-[2,2,2]-octane (DABCO)