Graphene enhanced electron transfer at aptamer modified electrode and its application in biosensing.
Graphene enhanced electron transfer at aptamer modified electrode and its application in biosensing.
复制标题
DOI：
10.1021/ac300521d
复制
发表时间：
2012-08
影响因子：
7.4
通讯作者：
Li Wang;Miao Xu;Lei Han;Ming Zhou;Chengzhou Zhu;S. Dong
中科院分区：
文献类型：
--
作者：
Li Wang;Miao Xu;Lei Han;Ming Zhou;Chengzhou Zhu;S. Dong
石墨烯（Graphene，GN）是一种单原子厚度的二维碳片，因其独特的形貌和性质而显示出令人兴奋的应用前景。本文利用GN的电子转移能力及其与ssDNA的独特相互作用，构建了一种新的电化学生物传感平台。将三磷酸腺苷结合适体（阿坝）固定在Au电极上，由于强的π-π相互作用，可使GN强烈吸附，从而使电极的电荷转移电阻（R（ct））大大降低。然而，阿坝与其靶点三磷酸腺苷（ATP）的结合反应抑制了GN的吸附，而R（ct）不能降低。在此基础上，我们开发了一种新的基于GN的小分子ATP生物传感平台。实验结果表明，所研制的电化学适配器对ATP具有良好的灵敏度和选择性。ATP的检测范围为15 × 10 ~（-9）~ 4 × 10 ~（-3）M。这里的方法是无标记和敏感的，不需要复杂的制造。此外，我们可以推广这种策略，以检测汞（2+）使用胸腺嘧啶（T）丰富，汞特异性寡核苷酸。因此，我们预计这种方法可以为设计高性能的电化学适配传感器提供一种有前途的方法，用于灵敏和选择性地检测一系列目标。
Graphene (GN), a two-dimensional and one-atom thick carbon sheet, is showing exciting applications because of its unique morphology and properties. In this work, a new electrochemical biosensing platform by taking advantage of the ultrahigh electron transfer ability of GN and its unique GN/ssDNA interaction was reported. Adenosine triphosphate binding aptamer (ABA) immobilized on Au electrode could strongly adsorb GN due to the strong π-π interaction and resulted in a large decrease of the charge transfer resistance (R(ct)) of the electrode. However, the binding reaction between ABA and its target adenosine triphosphate (ATP) inhibited the adsorption of GN, and R(ct) could not be decreased. On the basis of this, we developed a new GN-based biosensing platform for the detection of small molecule ATP. The experimental results confirmed that the electrochemical aptasensor we developed possessed a good sensitivity and high selectivity for ATP. The detection range for ATP was from 15 × 10(-9) to 4 × 10(-3) M. The method here was label-free and sensitive and did not require sophisticated fabrication. Furthermore, we can generalize this strategy to detect Hg(2+) using a thymine (T)-rich, mercury-specific oligonucleotide. Therefore, we expected that this method may offer a promising approach for designing high-performance electrochemical aptasensors for the sensitive and selective detection of a spectrum of targets.