Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation
Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation
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DOI：
10.1039/b905343n
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发表时间：
2009-01-01
影响因子：
3.1
通讯作者：
Di Mascio, Paolo
中科院分区：
文献类型：
--
作者：
Cadet, Jean;Douki, Thierry;Di Mascio, Paolo
这项调查旨在批判性地回顾关于UVA介导的细胞成分氧化反应的信息，重点是DNA作为主要光敏途径的结果。显然，在诱导氧化过程中，UVA辐射比UVB光子有效得多。报道了UVA诱导DNA氧化降解的主要途径，并从机理上进行了讨论。它们大多是根据单重态分子氧(O-1(2))的主要贡献和较小程度的羟基自由基((OH)-O-中心点)的贡献而合理化的，在后一种情况下，羟基自由基来自Fenton类型的反应。这导致在UVA辐射的细胞中形成主要的8-氧代-7，8-二氢鸟嘌呤，以及少量的氧化的嘧啶碱基和DNA链断裂。
The survey is aimed at critically reviewing information on the UVA-mediated oxidative reactions to cellular components with emphasis on DNA as the result of mostly photosensitized pathways. It appears clearly that UVA radiation is relatively much more efficient than UVB photons in inducing oxidative processes. The main UVA-induced oxidative degradation pathways of DNA are reported and discussed mechanistically. They are mostly rationalized in terms of a major contribution of singlet molecular oxygen (O-1(2)) and to a lesser extent of hydroxyl radical ((OH)-O-center dot), that in the latter case originates from Fenton-type reactions. This leads to the predominant formation of 8-oxo-7,8-dihydroguanine together with smaller amounts of oxidized pyrimidine bases and DNA strand breaks in UVA-irradiated cells.