The Direct Effects of Propofol on Pial Microvessels in Rabbits
The Direct Effects of Propofol on Pial Microvessels in Rabbits
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DOI：
10.1097/ana.0b013e31818b22d5
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发表时间：
2009-01-01
影响因子：
3.7
通讯作者：
Matsukawa, Takashi
中科院分区：
文献类型：
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作者：
Shibuya, Kazuhiro;Ishiyama, Tadahiko;Matsukawa, Takashi
异丙酚广泛用于神经外科麻醉；然而，它对软脑膜微血管系统的影响尚不清楚。因此，我们评估了异丙酚对兔子软脑膜微血管的直接影响。使用闭颅窗技术对 20 只日本白兔的软脑膜微循环进行可视化。在第一个实验（n = 14）中，在基线血流动力学测量后，颅窗灌注5个浓度递增的异丙酚（10（-8）、10（-7）、10（-6）、10（-5）、10（-4）mol/L：n = 8）或乳脂（在可比浓度'，n = 6）中溶解在人工脑脊液中7分钟每个。 5 μg/mL 的典型麻醉浓度相当于 10(-6) mol/L。第二个实验（n = 6）在戊巴比妥麻醉下局部涂抹去氧肾上腺素10(-6) mol/L和硝酸甘油10(-6) mol/L，持续7分钟。在第三个实验（n = 3）中，在戊巴比妥麻醉下测量脑电图和脑电双频指数。使用显微镜-视频捕获单元组合对选定的软脑膜小动脉和小静脉的直径进行可视化，然后使用数字视频分析仪进行测量。 10（-8）、10（-7）时局部应用异丙酚。 10(-6) 或10(-5) mol/L 不会改变软脑膜微血管的直径，然而，10(-4) mol/L 异丙酚会引起大动脉和小动脉扩张以及小静脉扩张。单独使用脂肪乳对血管直径没有任何显着影响。去氧肾上腺素和硝酸甘油分别使软脑膜小动脉和小静脉收缩和扩张。去氧肾上腺素收缩软脑膜小动脉和小静脉，硝酸甘油扩张软脑膜小动脉和小静脉。戊巴比妥既不产生爆发抑制，也不产生等电脑电图。结果证实了我们的假设：丙泊酚的临床相关浓度，即大约10(-6) mol/L，不会扩张软脑膜小动脉或小静脉。
Propofol is widely used for neurosurgical anesthesia; however, its effects on the pial microvasculature are unknown. We therefore evaluated the direct effects of propofol on pial microvessels in rabbits. Pial microcirculation was visualized using a closed cranial window technique in 20 Japanese white rabbits. In the first experiment (n = 14), after baseline hemodynamic measurements, the cranial window was superfused with 5 increasing concentrations of propofol (10(-8), 10(-7), 10(-6), 10(-5), 10(-4) mol/L: n = 8) or intralipid (at comparable concentrations', n = 6) dissolved in artificial cerebrospinal fluid for 7 Minutes each. A typical anesthetic concentration of 5 mu g/mL corresponds to 10(-6) mol/L. In the second experiment (n = 6), phenylephrine 10(-6) mol/L and nitroglycerin 10(-6) mol/L were applied topically for 7 minutes under pentobarbital anesthesia. In the third experiment (n = 3), electroencephalogram and bispectral index were measured under pentobarbital anesthesia. Diameters of selected pial arterioles and venules were visualized with a microscope-video capture unit combination and subsequently measured with a digital video analyzer. Topical application of propofol at 10(-8), 10(-7). 10(-6) or 10(-5) mol/L did not alter the diameters of the pial microvessels, however, at 10(-4) mol/L propofol induced dilation in large and small arterioles, along with venular dilation. Intralipid alone did not have any significant effect on vessel diameters. Phenylephrine and nitroglycerin produced pial arteriolar and venular constriction and dilation, respectively. Phenylephrine constricted and nitroglycerin dilated pial arterioles and venules. Pentobarbital did not produce either burst suppression or an isoelectric electro-encephalogram. The results confirm our hypothesis: clinically relevant concentrations of propofol, that is, approximately 10(-6) mol/L, do not dilate pial arterioles or venules.