A POLYSTYRENE MICROSPHERE ASSAY FOR DETECTING SURFACE HYDROPHOBICITY VARIATIONS WITHIN CANDIDA-ALBICANS POPULATIONS
A POLYSTYRENE MICROSPHERE ASSAY FOR DETECTING SURFACE HYDROPHOBICITY VARIATIONS WITHIN CANDIDA-ALBICANS POPULATIONS
复制标题
DOI：
10.1016/0167-7012(87)90066-2
复制
发表时间：
1987-07-01
影响因子：
2.2
通讯作者：
HAZEN, BW
中科院分区：
文献类型：
--
作者：
HAZEN, KC;HAZEN, BW
微生物病原体粘附到宿主细胞表面可能涉及疏水相互作用。在这里，我们描述了一种检测机会性真菌白色念珠菌群体和个体细胞表面疏水性的测定方法的发展。该试验包括将聚苯乙烯乳胶微球与细胞混合，随后枚举细胞附着的微球。用微球法和常用的水-烃双相分配法测定了酵母细胞群中类似水平的疏水性。发现各种缓冲液支持用微球法检测表面疏水性。复合真菌培养基则没有。试验培养基中的血清阻止微球治疗。与其他方法相比，该方法的一个独特优点是可以评估单个细胞的表面疏水性。在一个白色念珠菌酵母细胞群体中，观察到强烈、中度和弱疏水细胞。在一些母女细胞对中，只有一个细胞是疏水的。无论亲本细胞是否疏水，芽管和菌丝都是疏水的。结果表明，微球法是评价白色念珠菌细胞表面疏水性的有效方法。
Adherence of microbial pathogens to host cell surfaces may involve hydrophobic interactions. Here, we describe the development of an assay for detecting cell surface hydrophobicity of populations and individual cells of the opportunistic, fungal pathogen Candida albicans. The assay involves mixing polystyrene latex microspheres with cells and subsequent enumeration of cell-attached microspheres. Similar levels of hydrophobicity within a population of yeast cells were obtained with the microsphere assay and with a commonly used aqueous-hydrocarbon biphasic partitioning assay. Various buffers were found to support detection of surface hydrophobicity with the microsphere assay. Complex fungal growth media did not. Serum in test media prevented microsphere treatment. A unique advantage of the assay compared to others is that individual cells can be assessed for surface hydrophobicity. Within a population of C. albicans yeast cells, strongly, moderately and weakly hydrophobic cells were observed. Within some pairs of mother-daughter cells, only one cell was hydrophobic. Germ tubes and hyphae were hydrophobic regardless of the hydrophobic status of the parent cell. These results indicate that the microsphere assay is a useful test for evaluating cell surface hydrophobicity of C. albicans.