Role of nitric oxide in delaying senescence of cut rose flowers and its interaction with ethylene
Role of nitric oxide in delaying senescence of cut rose flowers and its interaction with ethylene
复制标题
一氧化氮延缓月季切花衰老的作用及其与乙烯的相互作用
DOI：
10.1016/j.scienta.2013.03.005
复制
发表时间：
2013-05-29
影响因子：
4.3
通讯作者：
Yu, Ji-Hua
中科院分区：
文献类型：
--
作者：
Liao, Wei-Biao;Zhang, Mei-Ling;Yu, Ji-Hua
近年来的研究表明，一氧化氮（NO）是植物体内一种重要的内源性信号分子。以月季切花为试材，研究了NO对切花衰老的调控作用。外源NO供体硝普钠（SNP）能延长瓶插寿命和最大花径，其效应具有剂量和时间依赖性。200 μ M SNP处理24 h，瓶插寿命和花径最长。NO清除剂2-（4-羧基2-苯基）-4，4，5，5-四甲基咪唑啉-1-氧基-3-氧化物（cPTIO）或亚甲蓝（MB-1）和NO合酶（NOS）抑制剂N-硝基-L-精氨酸甲酯（L-NAME）或S，S ′-1，3-亚苯基-双（1，2-乙二基）-双-异噻唑啉酮（PBITU）可逆转外源性NO的正效应，同时cPTIO、MB-1、L-NAME、或PBITU单独处理显著降低了切花的瓶插寿命，表明内源NO可能在切花衰老过程中起着特殊的作用。外源NO降低了1-氨基环丙烷-1-羧酸氧化酶（ACO）活性和乙烯产生量，cPTIO可拮抗NO的负效应，表明NO在月季切花衰老过程中可能起乙烯拮抗剂的作用。乙烯抑制剂1-甲基环丙烯（1-MCP）能提高月季切花的瓶插寿命和最大花径，但cPTIO、MB-1和L-NAME可部分逆转1-MCP的促进作用，表明内源NO在乙烯调控月季切花衰老中起重要作用。此外，1-MCP处理提高了切花月季叶片NOS活性和内源NO含量，抑制了ACO活性和乙烯释放。因此，NO可能通过抑制ACO活性来降低乙烯释放量，从而延长月季切花的瓶插寿命。这些结果表明，NO可能是乙烯调控切花月季衰老的信号分子。(C)2013爱思唯尔有限公司版权所有。
Recent studies showed that nitric oxide (NO) functions as an essential endogenous plant signal molecule. In this study, the regulatory role of NO during the flower senescence of cut rose (Rosa hybrida L 'Kardinal') was surveyed. Exogenous application of the NO donor sodium nitroprusside (SNP) could increase vase life and maximum flower diameter and its effects were dose and time dependent. Treatments with 200 mu M of SNP for 24 h obtained the maximum vase life and flower diameter. NO scavengers 2-(4-carboxy2-phenyl)-4,4, 5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) or methylene blue (MB-1) and NO synthase (NOS) inhibitors N-nitro-L-arginine methyl ester (L-NAME) or S,S'-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea (PBITU) reversed the positive effects of exogenous NO. Meanwhile, cPTIO, MB-1, L-NAME, or PBITU alone significantly decreased the vase life, indicating that endogenous NO may play specific roles in cut flower senescence. Exogenous NO decreased 1-aminocyclopro-pane-1-carboxylate oxidase (ACO) activity and ethylene production, and cPTIO was able to prevent the negative effects of NO. This implies that NO may act as an antagonist to ethylene in the senescence of cut rose flowers. Ethylene inhibitors 1-methylcyclopropene (1-MCP) improved vase life and maximum flower diameter, but the promotive effects of 1-MCP could be partially reversed by cPTIO, MB-1, or L-NAME, suggesting that endogenous NO in cut rose flowers may play a crucial role in the cut rose flowers senescence regulated by ethylene. Additionally, 1-MCP treatments increased NOS activity and endogenous NO production, while depressing ACO activity and ethylene production in cut rose. Thus, NO might decrease ethylene output by inhibiting ACO activity in cut rose flowers, thereby vase life being increased. Altogether, the results suggest that NO may function as a signal molecule involved in the senescence of cut rose regulated by ethylene. (C) 2013 Elsevier B.V. All rights reserved.