Origin of extracellular matrix synthesis during coronary repair.
Origin of extracellular matrix synthesis during coronary repair.
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冠状动脉修复过程中细胞外基质合成的起源。
DOI：
10.1161/01.cir.95.4.997
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发表时间：
1997
期刊：
影响因子：
37.8
通讯作者：
Zalewski,A
中科院分区：
文献类型：
--
作者：
Shi,Y;O'BrienJr,JE;Ala-Kokko,L;Chung,W;Mannion,JD;Zalewski,A
研究背景冠状动脉损伤后激活的外膜成纤维细胞向肌成纤维细胞分化，可能参与新生内膜形成和血管重塑。因此，本研究的目的是研究细胞外基质蛋白的合成增强的细胞起源，在冠状动脉repair.Methods和ResultsThe胶原和弹性蛋白的表达的时间过程和本地化进行了研究，通过原位杂交和免疫组织化学在猪冠状动脉球囊损伤后。损伤后2天内，外膜α 1（I）前胶原转录和细胞内I型前胶原蛋白表达增加。这是其次是持续合成的I型前胶原在新生内膜开始在7天和细胞外积累的I型胶原在两个层。合成细胞的起源进一步检查共定位的I型前胶原和溴脱氧尿苷标记激活的外膜细胞，易位到新生内膜。损伤后3个月，新生内膜细胞表现出持续的合成活性，表现为I型前胶原和弹性蛋白的存在。相反，媒体显示只有轻微的变化，在整个coronary repair.ConclusionsActivated外膜成纤维细胞合成胶原蛋白或弹性蛋白冠状动脉损伤后被赋予合成能力。它们表达I型前胶原，其中一些转移到内膜，在那里它们继续合成前胶原。 I型胶原蛋白的积聚在外膜和新生内膜中是明显的，而弹性蛋白主要积聚在新生内膜中。这些发现支持外膜成纤维细胞参与腔内损伤后的冠状动脉修复和重塑。
BackgroundCoronary injury triggers differentiation of activated adventitial fibroblasts to myofibroblasts, which may contribute to neointimal formation and vascular remodeling. Accordingly, the purpose of this study was to examine the cellular origin of the enhanced synthesis of extracellular matrix proteins during coronary repair.Methods and ResultsThe time course and localization of collagen and elastin expression were examined by in situ hybridization and immunohistochemistry in porcine coronary arteries after balloon-induced injury. Procollagen-α1(I) transcripts and intracellular type I procollagen protein increased in the adventitia within 2 days after injury. This was followed by a sustained synthesis of type I procollagen in neointima beginning at 7 days and the extracellular accumulation of type I collagen in both layers. The origin of synthetic cells was further examined by colocalization of type I procollagen and bromodeoxyuridine labeling to activated adventitial cells, which translocated to neointima. Neointimal cells exhibited sustained synthetic activity manifested by the presence of type I procollagen and elastin at 3 months after injury. In contrast, the media showed only minor changes in the synthesis of collagen or elastin throughout coronary repair.ConclusionsActivated adventitial fibroblasts are endowed with synthetic capabilities after coronary injury. They express type I procollagen, with some of them translocating to the intima, where they continue to synthesize procollagen. The accumulation of type I collagen is evident in the adventitia and neointima, whereas elastin accumulates mainly in neointima. These findings support the involvement of adventitial fibroblasts in coronary repair and remodeling after endoluminal injury.
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经导管输送 c-myc 反义寡聚物可减少猪冠状动脉球囊损伤模型中的新内膜形成
影响因子：
37.8
作者：
Yi Shi;A. Fard;Anthony Galeo;H. Hutchinson;Pawan Vermani;G. R. Dodge;D. Hall;F. Shaheen;A. Zalewski
通讯作者：
A. Zalewski
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DOI：
--
发表时间：
1992
期刊：
The American journal of pathology
影响因子：
--
作者：
Botney,MD;Kaiser,LR;Cooper,JD;Mecham,RP;Parghi,D;Roby,J;Parks,WC
通讯作者：
Parks,WC
影响因子：
20.1
作者：
BENDECK, MP;ZEMPO, N;REIDY, MA
通讯作者：
REIDY, MA
重塑而不是新生内膜形成解释了深部血管壁损伤后管腔狭窄：来自猪冠状动脉（再）狭窄模型的见解。
影响因子：
37.8
作者：
Henning Rud Andersen;Michael Maeng;M. Thorwest;Erling Falk
通讯作者：
Erling Falk
兔抗肾小球基底膜抗体诱导的新月体肾炎中血管外膜细胞表达 I 型胶原信使核糖核酸。
DOI：
--
发表时间：
1993
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Wiggins,R;Goyal,M;Merritt,S;Killen,PD
通讯作者：
Killen,PD