Biodegradation of pyrazosulfuron-ethyl by Acinetobacter sp. CW17
Biodegradation of pyrazosulfuron-ethyl by Acinetobacter sp. CW17
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DOI：
10.1007/s12223-012-0107-8
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发表时间：
2012-03
影响因子：
2.6
通讯作者：
Yan-hui Wang;L. Du;Yingxi Chen;Xiaoliang Liu;Xiaomao Zhou;Huihua Tan;L. Bai;Dongqiang Zeng
中科院分区：
文献类型：
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作者：
Yan-hui Wang;L. Du;Yingxi Chen;Xiaoliang Liu;Xiaomao Zhou;Huihua Tan;L. Bai;Dongqiang Zeng
从长沙市磺隆工厂仓库附近的土壤中分离到一株磺隆降解菌CW 17。经鉴定，菌株CW 17为不动杆菌属（Acinetobacterium sp.）基于94碳源利用或Biolog微孔板中的化学敏感性、常规表型特征和16 S rRNA基因测序的分析。以乙嘧磺隆为唯一碳源，考察了乙嘧磺隆浓度、pH和温度对降解的影响。初始浓度为5.0、20.0和50.0 mg/L的嘧磺隆在接种7 d后的降解率分别为48.0%、77.0%和32.6%。该菌株的生长在低pH缓冲液中受到抑制。化学降解在低pH下比在中性和碱性pH条件下发生得快得多。CW 17菌株在30°C下对乙嘧磺隆的降解速率比20 ° C和37°C下的降解速率快。通过液相色谱-质谱（LC/MS）分析了降解的两种代谢产物。结果表明，菌株CW 17能够通过磺酰脲桥的断裂来降解乙嘧磺隆。
The pyrazosulfuron-ethyl-degrading bacterium, designated as CW17, was isolated from contaminated soil near the warehouse of the factory producing pyrazosulfuron-ethyl in Changsha city, China. The strain CW17 was identified asAcinetobactersp. based on analyses of 94 carbon source utilization or chemical sensitivity in Biolog microplates, conventional phenotypic characteristics, and 16S rRNA gene sequencing. When pyrazosulfuron-ethyl was provided as the sole carbon source, the effects of pyrazosulfuron-ethyl concentration, pH, and temperature on biodegradation were examined. The degradation rates of pyrazosulfuron-ethyl at initial concentrations of 5.0, 20.0, and 50.0 mg/L were 48.0%, 77.0%, and 32.6%, respectively, after inoculation for 7 days. The growth of the strain was inhibited at low pH buffers. The chemical degradation occurs much faster at low pH than at neutral and basic pH conditions. The degradation rate of pyrazosulfuron-ethyl at 30°C was faster than those at 20 and 37°C by CW17 strains. Two metabolites of degradation were analyzed by liquid chromatography–mass spectroscopy (LC/MS). Based on the identified products, strain CW17 seemed to be able to degrade pyrazosulfuron-ethyl by cleavage of the sulfonylurea bridge.