Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells
Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells
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DOI：
10.1139/o05-063
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发表时间：
2005-10-01
影响因子：
2.9
通讯作者：
Hurta, RAR
中科院分区：
文献类型：
--
作者：
Matchett, MD;MacKinnon, SL;Hurta, RAR
基质金属蛋白酶（matrix metalloproteinases，MMPs）是细胞外基质（extracellular matrix，ECM）降解的主要介质，其调节对于调节ECM蛋白水解是至关重要的，ECM蛋白水解在转移中是重要的。本研究考察了3种富含类黄酮的组分（粗组分。花青素苷富集级分和原花青素富集级分）对DU 145人前列腺癌细胞中MMP活性的体外影响。使用明胶凝胶电泳，MMP活性进行了评估后，24小时暴露于蓝莓馏分的细胞。所有馏分引起的能力，降低MMP-2和MMP-9的活性。在测试的组分中，发现富含原花色素的组分在抑制这些细胞中的MMP活性方面是最有效的。在这些细胞中没有观察到响应于用蓝莓级分处理的坏死或凋亡细胞死亡的诱导。这些发现表明蓝莓黄酮类化合物具有有效降低MMP活性的能力，这可能会降低整体ECM降解。这种能力在控制肿瘤转移形成中可能是重要的。
Regulation of the matrix metalloproteinases (MMPs), the major mediators of extracellular matrix (ECM) degradation, is crucial to regulate ECM proteolysis, which is important in metastasis. This study examined the effects of 3 flavonoid-enriched fractions (a crude fraction. an anthocyanin-enriched fraction, and a proanthocyanidin-enriched fraction), which were prepared from lowbush blueberries (Vaccinium angustifolium), on MMP activity in DU145 human prostate cancer cells in vitro. Using gelatin gel electrophoresis, MMP activity was evaluated from cells after 24-hr exposure to blueberry fractions. All fractions elicited an ability to decrease the activity of MMP-2 and MMP-9. Of the fractions tested, the proanthocyanidin-enriched fraction was found to be the most effective at inhibiting MMP activity in these cells. No induction of either necrotic or apoptotic cell death was noted in these cells in response to treatment with the blueberry fractions. These findings indicate that flavonoids from blueberry possess the ability to effectively decrease MMP activity, which may decrease overall ECM degradation. This ability may be important in controlling tumor metastasis formation.