Switchable Solvatochromic Probes for Live-Cell Super-resolution Imaging of Plasma Membrane Organization
Switchable Solvatochromic Probes for Live-Cell Super-resolution Imaging of Plasma Membrane Organization
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DOI：
10.1002/anie.201907690
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发表时间：
2019-08-27
影响因子：
16.6
通讯作者：
Klymchenko, Andrey S.
中科院分区：
文献类型：
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作者：
Danylchuk, Dmytro, I;Moon, Seonah;Klymchenko, Andrey S.
由于缺乏适当的荧光探针，细胞膜纳米级组织的可视化仍然具有挑战性。在这里，我们介绍了一种新的设计概念，结合特定的膜靶向，开/关开关，和环境传感功能的超分辨率显微镜探针。提出了一种提高溶剂化变色染料尼罗红光稳定性的功能化策略。染料接枝到一个新开发的膜靶向部分组成的磺酸酯基团和不同长度的烷基链。虽然具有强膜结合的长链探针NR 12 A适用于常规显微镜，但由于可逆结合，短链探针NR 4A能够在活细胞表面独家进行脂质顺序的第一次纳米级制图。后者的探针揭示了存在的纳米级的突起和内陷的较低的脂质顺序在质膜，这表明膜形态和脂质组织之间的微妙的连接。
Visualization of the nanoscale organization of cell membranes remains challenging because of the lack of appropriate fluorescent probes. Herein, we introduce a new design concept for super-resolution microscopy probes that combines specific membrane targeting, on/off switching, and environment sensing functions. A functionalization strategy for solvatochromic dye Nile Red that improves its photostability is presented. The dye is grafted to a newly developed membrane-targeting moiety composed of a sulfonate group and an alkyl chain of varied lengths. While the long-chain probe with strong membrane binding, NR12A, is suitable for conventional microscopy, the short-chain probe NR4A, owing to the reversible binding, enables first nanoscale cartography of the lipid order exclusively at the surface of live cells. The latter probe reveals the presence of nanoscopic protrusions and invaginations of lower lipid order in plasma membranes, suggesting a subtle connection between membrane morphology and lipid organization.