Cellular changes during repair of a cryogenic spinal cord injury in the rat: an electron microscopic study.
Cellular changes during repair of a cryogenic spinal cord injury in the rat: an electron microscopic study.
复制标题
大鼠低温脊髓损伤修复过程中的细胞变化：电子显微镜研究。
DOI：
10.1097/00005072-198901000-00008
复制
发表时间：
1989
影响因子：
3.2
通讯作者：
Collins,GH
中科院分区：
文献类型：
--
作者：
West,NR;Collins,GH
在受控非侵入性条件下建立成年大鼠脊髓低温损伤模型，研究组织连续性和完整性相对保持的成年哺乳动物中枢神经组织的修复与再生。在这些实验条件下，轴突和髓鞘被破坏，胶质细胞的基质被保留，轴突的再生明显。损伤后7天、15天、30天和60天的电子显微镜观察显示轴突结构显示再生，星形细胞结构似乎为基质和轴突提供支持，以及少突胶质细胞和雪旺细胞的轴突髓鞘形成。这些细胞事件恢复了损伤区域内的大部分正常结构，直到其与沃勒带的交界处。在这个交界区，形态证据可能表明，即使在60天后，轴突、星形胶质细胞和髓鞘细胞仍有持续的细胞活动。这些研究表明，在理想的条件下，受损的轴突可能能够在成年哺乳动物的中枢神经系统内再生，这个模型提供了一个机会来定义一些机制。
Cryogenic injury of adult, rat spinal cord was produced under controlled non-invasive conditions to study repair and regeneration in adult, mammalian central nervous tissue in which tissue continuity and integrity are relatively preserved. Under these experimental conditions axons and myelin are destroyed, a matrix of glial cells is preserved, and regrowth of axons is apparent. Electron microscopic studies at 7, 15, 30 and 60 days post-injury demonstrate axonal structures indicative of regrowth, astrocytic structures which appear to provide support to both matrix and axons and myelination of axons by both oligodendrocytes and Schwann cells. These cellular events restore much of the normal structure within the injured area up to its junction with the Wallerian zone. In this junctional zone morphologic evidence may indicate continuing cellular activity, even at 60 days, in axons, astrocytes and myelinating cells. These studies suggest that under ideal conditions damaged axons may be capable of regeneration within the adult mammalian central nervous system and that this model provides an opportunity to define some of the mechanisms.