Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes.
Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes.
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鼠胚胎干细胞衍生的心肌细胞的全球转录组分析。
DOI：
10.1186/gb-2007-8-4-r56
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Sachinidis, Agapios
中科院分区：
文献类型：
--
作者：
Doss, Michael Xavier;Winkler, Johannes;Chen, Shuhua;Hippler-Altenburg, Rita;Sotiriadou, Isaia;Halbach, Marcel;Pfannkuche, Kurt;Liang, Huamin;Schulz, Herbert;Hummel, Oliver;Huebner, Norbert;Rottscheidt, Ruth;Hescheler, Juergen;Sachinidis, Agapios
Microarray analysis reveals that the specific pattern of gene expression in cardiomyocytes derived from embryonic stem cells reflects the biological, physiological and functional processes occurring in mature cardiomyocytes. Characterization of gene expression signatures for cardiomyocytes derived from embryonic stem cells will help to define their early biologic processes. A transgenic α-myosin heavy chain (MHC) embryonic stem cell lineage was generated, exhibiting puromycin resistance and expressing enhanced green fluorescent protein (EGFP) under the control of the α-MHC promoter. A puromycin-resistant, EGFP-positive, α-MHC-positive cardiomyocyte population was isolated with over 92% purity. RNA was isolated after electrophysiological characterization of the cardiomyocytes. Comprehensive transcriptome analysis of α-MHC-positive cardiomyocytes in comparison with undifferentiated α-MHC embryonic stem cells and the control population from 15-day-old embryoid bodies led to identification of 884 upregulated probe sets and 951 downregulated probe sets in α-MHC-positive cardiomyocytes. A subset of upregulated genes encodes cytoskeletal and voltage-dependent channel proteins, and proteins that participate in aerobic energy metabolism. Interestingly, mitosis, apoptosis, and Wnt signaling-associated genes were downregulated in the cardiomyocytes. In contrast, annotations for genes upregulated in the α-MHC-positive cardiomyocytes are enriched for the following Gene Ontology (GO) categories: enzyme-linked receptor protein signaling pathway (GO:0007167), protein kinase activity (GO:0004672), negative regulation of Wnt receptor signaling pathway (GO:0030178), and regulation of cell size (O:0008361). They were also enriched for the Biocarta p38 mitogen-activated protein kinase signaling pathway and Kyoto Encyclopedia of Genes and Genomes (KEGG) calcium signaling pathway. The specific pattern of gene expression in the cardiomyocytes derived from embryonic stem cells reflects the biologic, physiologic, and functional processes that take place in mature cardiomyocytes. Identification of cardiomyocyte-specific gene expression patterns and signaling pathways will contribute toward elucidating their roles in intact cardiac function.
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DOI：
10.1083/jcb.143.7.2045
发表时间：
1998-12-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kolossov E;Fleischmann BK;Liu Q;Bloch W;Viatchenko-Karpinski S;Manzke O;Ji GJ;Bohlen H;Addicks K;Hescheler J
通讯作者：
Hescheler J
影响因子：
--
作者：
Sachinidis, A;Gissel, C;Hescheler, J
通讯作者：
Hescheler, J
影响因子：
6.7
作者：
Gissel, C;Voolstra, C;Sachinidis, A
通讯作者：
Sachinidis, A
DOI：
10.1083/jcb.111.6.2427
发表时间：
1990-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lyons GE;Schiaffino S;Sassoon D;Barton P;Buckingham M
通讯作者：
Buckingham M
影响因子：
15.9
作者：
Klug, MG;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ