Morphological and Functional Characteristics of Acinar Atrophy and Recovery in the Duct-ligated Parotid Gland of the Rat
Morphological and Functional Characteristics of Acinar Atrophy and Recovery in the Duct-ligated Parotid Gland of the Rat
复制标题
大鼠腮腺结扎腺腺泡萎缩与恢复的形态和功能特征
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Peter M. Smith
中科院分区：
文献类型：
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作者：
John Scott;P. Liu;Peter M. Smith
尽管腺泡萎缩在唾液腺疾病中经常发生，但结构变化和功能下降之间的关系尚未明确，并且组织学和功能完整性恢复的潜力尚未完全量化。因此，我们的目的是进一步加深对病理性腺泡萎缩的理解。斯坦森的导管被结扎了长达六周的时间，并且在单独的实验中，两周后被解除结扎并允许恢复长达两周。进行定性和定量组织学分析。此外，还测量了来自结扎和去结扎腺体的酶促分散细胞对神经激素刺激的反应能力。结果证实，完全阻塞的腺体会经历快速、进行性的严重萎缩，两周内超过 85% 的腺泡组织绝对消失。腺泡萎缩和细胞凋亡导致的细胞损失是腺体萎缩的原因。剩余的小叶内上皮由极度萎缩的腺泡和许多中间形式的导管样结构组成。来自萎缩腺体的分散细胞表现出与正常情况相似的激动剂诱导的氯化物释放。总之，这些结构功能结果证实了阻塞腺体中腺泡样细胞的持续活力，并表明导管样结构源自幸存的萎缩性腺泡。两周后，脱结扎的腺体表现出接近正常的结构恢复。它们的酶促分散细胞对激动剂刺激有正常反应。结果支持这样的观点，即病理性萎缩与生理性萎缩在很大程度上相似，提供了腺泡细胞在不利条件下生存的机制，并有最终恢复的可能性。
Although acinar atrophy occurs frequently in salivary diseases, the relationship between structural changes and functional decrements is not well-established, and the potential for recovery of histological and functional integrity has not been wholly quantified. We aimed, therefore, to develop further our understanding of pathological acinar atrophy. Stensen's duct was ligated for periods up to six weeks and, in separate experiments, was de-ligated after two weeks and allowed to recover for up to two weeks. Qualitative and quantitative histological analyses were carried out. Additionally, the ability of enzymatically dispersed cells from ligated and de-ligated glands to respond to neuro-hormonal stimuli was also measured. The results confirmed that totally obstructed glands undergo a rapid, progressive severe atrophy amounting to absolute losses of over 85% of acinar tissue by two weeks. Acinar shrinkage and cell losses through apoptosis accounted for the glandular atrophy. Remaining intralobular epithelia consisted of extremely atrophic acini and numerous duct-like structures with intermediate forms. Dispersed cells from atrophic glands exhibited agonist-induced release of chloride similar to normal. Together, these structural-functional results confirm the persistent viability of acinar-like cells in the obstructed gland and suggest that the duct-like structures are derived from surviving atrophic acini. De-ligated glands exhibited a near-normal recovery of structure by two weeks. Their enzymatically dispersed cells responded normally to agonist stimulation. The results support the view that pathological atrophy is largely similar to physiological atrophy, providing a mechanism for acinar cell survival under adverse conditions, with the possibility of eventual recovery.
影响因子：
6.7
作者：
A. Haimovitz-Friedman;R. Kolesnick;Z. Fuks
通讯作者：
A. Haimovitz-Friedman;R. Kolesnick;Z. Fuks