Differential miRNA expression profiling reveals a correlation between hbr-miR156 and laticifer differentiation in rubber trees
Differential miRNA expression profiling reveals a correlation between hbr-miR156 and laticifer differentiation in rubber trees
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差异 miRNA 表达谱揭示橡胶树中 hbr-miR156 与乳管分化之间的相关性
DOI：
10.1016/j.indcrop.2022.116067
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发表时间：
2022-12-06
影响因子：
5.9
通讯作者：
Tian，Wei-Min
中科院分区：
文献类型：
--
作者：
Wu，Shaohua;Zhang，Shixin;Tian，Wei-Min
微RNA(MiRNA)在植物的发育和形态发生中起着关键作用，因为它可以参与靶基因的切割和翻译抑制。Laticifer是橡胶树中一种特殊的组织，用于橡胶的自然生物合成和储存，它有别于维管形成层中的纺锤形首字母。然而，目前关于miRNAs参与乳管分化的知识有限。本研究利用Illumina HiSeq-2000技术，获得了茉莉酸(JA)的模拟物冠状病毒(Coronatine，COR)对形成层区域miRNA的差异表达谱。根据miRBase数据库(版本22.1)和CATAS7-33-97基因组，使用Mireap_v0.2软件共鉴定了441个已知成熟miRNAs和839个新的成熟miRNAs。其中119个miRNAs在COR反应的早期和晚期表现出差异表达，并且在两个阶段的miRNAs表达存在差异。Hbr-miR156的过表达导致俄罗斯蒲公英(Taraxacum Kok-saghyz.Rodin)的乳管细胞数量显著减少。HBR-miR156可能参与了冠状病毒诱导的乳管分化，为miRNAs如何调控维管形成层向乳管细胞的分化提供了新的视角。
A microRNA (miRNA) play a pivotal role in plant development and morphogenesis, as it can be involved in the cleavage of target genes and translational repression. Laticifer is a specific tissue in rubber trees that is used in the natural biosynthesis and storage of rubber and it differentiates from fusiform initials in the vascular cambia. However, current knowledge about the involvement of miRNAs in laticifer differentiation is limited. In the present study, the differential miRNA expression profiles in the cambial regions in response to coronatine (COR), a jasmonic acid (JA) mimic, were obtained using Illumina HiSeq-2000 technology. A total of 441 known mature miRNAs and 839 novel mature miRNAs were identified using the Mireap_v0.2 software, according to the miRBase database (Release 22.1) and CATAS7–33–97 genome. Of which, 119 miRNAs exhibited differential expression during the early and late-stage responses to COR, and the miRNAs differed between the two stages. Overexpression of hbr-miR156 caused a significant decrease in the number of laticifer cells in Russian dandelion (Taraxacum kok-saghyz.Rodin). The hbr-miR156 may be involved in coronatine-induced laticifer differentiation, providing a new perspective as to how miRNAs regulate the differentiation of the vascular cambia into laticifer cells.