Genome sequence of Halomonas hydrothermalis Y2, an efficient ectoine-producer isolated from pulp mill wastewater.
Genome sequence of Halomonas hydrothermalis Y2, an efficient ectoine-producer isolated from pulp mill wastewater.
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DOI：
10.1016/j.jbiotec.2018.08.017
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发表时间：
2018-11
影响因子：
4.1
通讯作者：
Qi Zhao;Yiwei Meng;Shannan Li;Pei-jun Lv;P. Xu;Chunyu Yang
中科院分区：
文献类型：
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作者：
Qi Zhao;Yiwei Meng;Shannan Li;Pei-jun Lv;P. Xu;Chunyu Yang
嗜盐微生物在生物工程中具有巨大的应用潜力，嗜盐单胞菌（HalomonashhydrothermalisY 2）是从高Na+制浆废水中分离到的一株耐盐、嗜碱的菌株。该菌株在造纸废水中占优势，并在利用各种底物方面表现出代谢多样性。在这里，我们提出了该菌株的基因组序列，其中包括一个环形染色体大小为3，933，432 bp，GC含量为60.2%。从基因组中鉴定出编码相容性溶质合成和转运蛋白的不同基因。该菌株具有完整的四氢嘧啶合成途径，可将谷氨酸转化为四氢嘧啶并部分分泌到培养基中。此外，通过删除四氢嘧啶羟化酶（EctD），增加了约20%的四氢嘧啶。我们在这里报告的基因组序列将提供关于菌株Y2对其恶劣栖息地的适应机制的遗传信息，以及促进探索不同相容溶质的代谢策略，例如，四氢嘧啶生产。
Halophilic microorganisms have great potentials towards biotechnological applications.Halomonas hydrothermalisY2 is a halotolerant and alkaliphilic strain that isolated from the Na+-rich pulp mill wastewater. The strain is dominant in the bacterial community of pulp mill wastewater and exhibits metabolic diversity in utilizing various substrates. Here we present the genome sequence of this strain, which comprises a circular chromosome 3,933,432 bp in size and a GC content of 60.2%. Diverse genes that encoding proteins for compatible solutes synthesis and transport were identified from the genome. With a complete pathway for ectoine synthesis, the strain could produce ectoine from monosodium glutamate and further partially secreted into the medium. In addition, around 20% ectoine was increased by deleting the ectoine hydroxylase (EctD). The genome sequence we report here will provide genetic information regarding adaptive mechanisms of strain Y2 to its harsh habitat, as well as facilitate exploration of metabolic strategies for diverse compatible solutes,e.g., ectoine production.