Time-lapse mesoscopy of Candida albicans and Staphylococcus aureus dual-species biofilms reveals a structural role for the hyphae of C. albicans in biofilm formation
Time-lapse mesoscopy of Candida albicans and Staphylococcus aureus dual-species biofilms reveals a structural role for the hyphae of C. albicans in biofilm formation
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DOI：
10.1101/2023.08.31.555792
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发表时间：
2023-09
期刊：
影响因子：
1.5
通讯作者：
K. Baxter;Fiona A. Sargison;J. R. Fitzgerald;Gail McConnell;P. Hoskisson
中科院分区：
文献类型：
--
作者：
K. Baxter;Fiona A. Sargison;J. R. Fitzgerald;Gail McConnell;P. Hoskisson
白色念珠菌和金黄色葡萄球菌的多微生物感染可能导致伴随的毒力和抗微生物药物耐药性的增加。这种增强的致病性表型是由多种因素介导的，包括代谢过程和金黄色葡萄球菌与白色念珠菌菌丝的直接相互作用。已知生物膜的整体结构有助于它们对治疗的抵抗，然而物种之间直接相互作用的动力学以及它如何促进致病性却知之甚少。为了解决这个问题，开发了一种新的延时介观光学成像方法，使白色念珠菌/S的形成成为可能。金黄色葡萄球菌全双种生物膜有待跟进。研究发现，随着生物膜的成熟，酵母型和菌丝型白色念珠菌对生物膜的结构有深远的影响。由于不同的白色念珠菌形态，在每个生物膜内产生不同的白色念珠菌和金黄色葡萄球菌亚群，从而产生不同的亚区。这些数据表明，白色念珠菌细胞形态在整体生物膜结构的发展和菌落宏观结构的出现中起着关键作用，并可能暂时影响感染中的协同作用。
Polymicrobial infection with Candida albicans and Staphylococcus aureus may result in a concomitant increase in virulence and resistance to antimicrobial drugs. This enhanced pathogenicity phenotype is mediated by numerous factors including metabolic processes and direct interaction of S. aureus with C. albicans hyphae. The overall structure of biofilms is known to contribute to their recalcitrance to treatment, however the dynamics of direct interaction between species and how it contributes to pathogenicity is poorly understood. To address this, a novel time-lapse mesoscopic optical imaging method was developed to enable the formation of C. albicans/S. aureus whole dual-species biofilms to be followed. It was found that yeast-form or hyphal-form C. albicans in the biofilm founder-population profoundly affects the structure of the biofilm as it matures. Different sub-populations of C. albicans and S. aureus arise within each biofilm as a result of the different C. albicans morphotypes, resulting in distinct sub-regions. These data reveal that C. albicans cell morphology is pivotal in the development of global biofilm architecture and the emergence of colony macrostructures and may temporally influence synergy in infection.