A nanoparticle co‐matrix for multiple charging in matrix‐assisted laser desorption ionization imaging of tissue
A nanoparticle co‐matrix for multiple charging in matrix‐assisted laser desorption ionization imaging of tissue
复制标题
用于在基质中多次充电的纳米颗粒共基质辅助激光解吸电离组织成像
DOI：
10.1002/rcm.8424
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发表时间：
2019
影响因子：
2
通讯作者：
Murray, Kermit K.
中科院分区：
文献类型：
--
作者：
Banstola, Bijay;Murray, Kermit K.
RationaleA由2 -硝基间苯三酚（2 - NPG）和二氧化硅纳米颗粒组成的双组分基质用于基质辅助激光解吸电离（MALDI）质谱成像组织中高电荷态生物分子。潜在的优势包括增加有效质量范围和破碎效率。方法将2‐NPG基质与纳米二氧化硅的混合物应用于10 μm厚的小鼠脑组织。将混合物移液到组织上进行分析，并喷射用于组织成像。MALDI图像是用商用飞行时间质谱仪在高真空条件下获得的。结果2 - NPG和纳米颗粒复合基质在高真空MALDI组织中产生高电荷离子。对直径为20,70,400和1000nm的纳米颗粒进行了测试，其中20nm颗粒产生的电荷态最高。从高电荷离子获得的小鼠脑组织图像显示出类似的空间定位。结论2 - NPG和纳米颗粒结合的基质可以从组织中产生高电荷离子，其机制可能依赖于颗粒的高表面积，可以使组织干燥，并且它们能够结合分析物分子，从而帮助晶体形成和在激光照射下产生多电荷离子。
RationaleA two‐component matrix of 2‐nitrophloroglucinol (2‐NPG) and silica nanoparticles was used for matrix‐assisted laser desorption ionization (MALDI) mass spectrometry imaging of high‐charge‐state biomolecules in tissue. Potential advantages include increased effective mass range and efficiency of fragmentation.MethodsA mixture of 2‐NPG matrix and silica nanoparticles was applied to cyrosectioned 10 μm thick mouse brain tissue. The mixture was pipetted onto the tissue for profiling and sprayed for tissue imaging. MALDI images were obtained under high vacuum in a commercial time‐of‐flight mass spectrometer.ResultsThe combined 2‐NPG and nanoparticle matrix produced highly charged ions from tissue with high‐vacuum MALDI. Nanoparticles of 20, 70, 400, and 1000 nm in diameter were tested, the 20 nm particles producing the highest charge states. Images of mouse brain tissue obtained from highly charged ions show similar spatial localization.ConclusionsThe combined 2‐NPG and nanoparticle matrix produces highly charged ions from tissue through a mechanism that may rely on the high surface area of the particles which can dry the tissue, and their ability to bind analyte molecules thereby assisting in crystal formation and production of multiply charged ions on laser irradiation.