Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells.
Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells.
复制标题
培养细胞中染色体分离的实时荧光成像。
DOI：
10.1007/978-1-0716-1904-9_5
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Gorbsky,GaryJ
中科院分区：
文献类型：
--
作者：
Daum,JohnR;DuBose,CaseyO;Sivakumar,Sushama;Gorbsky,GaryJ
活细胞荧光显微镜是一种有效的工具，用于表征暴露于化学抑制剂和RNA干扰介导或CRISPR敲除介导的蛋白靶点耗竭后产生的异常有丝分裂表型。有丝分裂过程中培养细胞的实时成像在维持细胞最佳健康的同时实现时间和空间分辨率以实现研究目标方面提出了挑战。本文介绍了利用宽视场或光片倒置荧光显微镜监测和分析哺乳动物细胞有丝分裂的策略。
Live-cell fluorescence microscopy is an effective tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors and after RNA interference-mediated or CRISPR knockout-mediated depletion of protein targets. Live imaging of cultured cells during mitotic progression presents challenges in maintaining optimal health of cells while achieving the temporal and spatial resolution to accomplish the goals of the study. Herein are strategies to monitor and analyze mammalian cell mitosis utilizing either a wide field or a light sheet, inverted, fluorescence microscope.