Expression of B2-type cyclin-dependent kinase is controlled by protein degradation in Arabidopsis thaliana
Expression of B2-type cyclin-dependent kinase is controlled by protein degradation in Arabidopsis thaliana
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DOI：
10.1093/pcp/pcl034
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发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Umeda, Masaaki
中科院分区：
文献类型：
--
作者：
Adachi, Sumiko;Uchimiya, Hirofumi;Umeda, Masaaki
真核细胞周期由细胞周期蛋白依赖性激酶（CDKs）控制。植物细胞周期蛋白激酶有6种类型，其中B型细胞周期蛋白激酶（CDKB）在S-晚期至M-期特异性表达。我们证明了拟南芥CDKB 2的表达是在蛋白质降解机制的控制下。与CDKB 2的假定PEST基序融合的β-葡萄糖醛酸酶在烟草Bright Yellow-2细胞和拟南芥植物中是不稳定的，并且其降解被蛋白酶体抑制剂MG 132阻止。我们认为CDKB 2蛋白的丰度不仅在转录水平上受到调节，而且还通过蛋白酶体介导的蛋白降解来调节。
The eukaryotic cell cycle is controlled by cyclin-dependent kinases (CDKs). Plants possess six types of CDK, among which the B-type CDK (CDKB) is expressed specifically from the late S- to the M-phase. We demonstrate that the expression of Arabidopsis CDKB2 is under the control of the protein degradation machinery. beta-Glucuronidase fused to a putative PEST motif of CDKB2 was unstable in tobacco Bright Yellow-2 cells and Arabidopsis plants, and its degradation was arrested by the proteasome inhibitor MG132. We propose that the abundance of CDKB2 protein is regulated not only at the transcriptional level, but also through proteasome-mediated protein degradation.