Odor-induced variation in anxiety-like behavior in mice is associated with discrete and differential effects on mesocorticolimbic cholecystokinin mRNA expression
Odor-induced variation in anxiety-like behavior in mice is associated with discrete and differential effects on mesocorticolimbic cholecystokinin mRNA expression
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DOI：
10.1016/s0893-133x(02)00354-8
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发表时间：
2002-11-01
影响因子：
7.6
通讯作者：
Drolet, G
中科院分区：
文献类型：
--
作者：
Hebb, ALO;Zacharko, RM;Drolet, G
本研究评估了CD-I小鼠在新的捕食者和非捕食者气味暴露和明暗测试后中皮质边缘胆囊收缩素（CCK） mRNA的变化。简而言之，CD-I小鼠急性暴露于捕食者气味2,5-二氢- 2,4,5 -三甲基噻唑啉（TMT；红狐肛门腺分泌物的主要成分）或对照气味丁酸（BA）中，在气味呈现期间抑制了饲养行为，随后在明暗测试中引起焦虑，并且与生理盐水处理小鼠相比，中皮质边缘CCK mRNA增加有关。只有暴露于TMT的小鼠在气味处理期间表现出更高的冻结行为。在明暗测试中，与暴露于生理盐水的小鼠相比，暴露于BA或TMT的小鼠需要更长的时间才能重新进入仪器的光区，并且在光中花费的累积时间更短。在暴露于狐狸气味的小鼠中，光和光暗转换时间的减少被夸大了。气味表现与中皮质边缘部位CCK mRNA的增加有关。丁酸与VTA中CCK基因表达增强相关，而BA和TMT均与内侧前额叶皮层（mPFC） CCK mRNA水平升高相关。尽管在明暗箱中进行了测试，但在小鼠中，VIA和mPFC内CCK mRNA的增加是明显的。相比之下，光暗实验小鼠的杏仁核基底外侧核（BLA） CCK mRNA在气味暴露后增强，而盐水处理小鼠的BLA CCK mRNA在光暗实验后自然降低。CCK mRNA的差异模式与离散的心因性应激源操作和焦虑样行为的挑衅相关的经验进行了讨论。(C) 2002美国神经精神药理学学会。Elsevier Science Inc.出版。
The present investigation assessed alterations in mesocorticolimbic cholecystokinin (CCK) mRNA following novel predator and non-predator odor exposure and light-dark testing in CD-I mice. In brief, acute exposure of CD-I mice to the predator odor, 2,5-dihydro-2, 4,5-trimethylthiazoline (TMT; the major component of the anal gland secretions of the red fox), or the control odor, butyric acid (BA), suppressed rearing behavior during odor presentation, subsequently induced anxiety in the light dark test, and was associated with increased mesocorticolimbic CCK mRNA relative to saline treated mice. Only mice exposed to TMT displayed elevated freezing behaviors during odor treatment. In the light-dark test, mice exposed to either BA or TMT took longer to reenter the light section of the apparatus and spent less cumulative time in the light relative to mice exposed to saline. The decreased time spent in the light as well as light dark transitions were exaggerated among mice exposed to fox odor. Odor presentation was associated with increased CCK mRNA in mesocorticolimbic sites. Butyric acid was associated with enhanced CCK gene expression in the VTA, while both BA and TMT were associated with increased medial prefrontal cortex (mPFC) CCK mRNA levels. Increased CCK mRNA within the VIA and mPFC was evident among mice despite testing in the light-dark box. In contrast, basolateral nucleus of the amygdala (BLA) CCK mRNA was enhanced following Odor exposure among mice in the light dark test relative only to saline treated mice which demonstrated a natural decrease in BLA CCK mRNA following the light dark test. The differential pattern of CCK mRNA associated with discrete psychogenic stressor manipulations and the provocation of anxiety-like behavior associated with such experiences is discussed. (C) 2002 American College of Neuropsychopharmacology. Published by Elsevier Science Inc.