SEC of mono-carboxymethyl cellulose (CMC) an a wide range of pH; Mark-Houwink constants
SEC of mono-carboxymethyl cellulose (CMC) an a wide range of pH; Mark-Houwink constants
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DOI：
10.1016/s0144-8617(97)00259-2
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发表时间：
1998-08-01
影响因子：
11.2
通讯作者：
Bykova, TO
中科院分区：
文献类型：
--
作者：
Eremeeva, TE;Bykova, TO
开发了一种使用双检测器 HPSEC（带折射计的在线紫外检测器）测定羧甲基纤维素 (CMC) 分子量 (M-w) 和化学异质性（氧化度 (DO)）的方法。已发现使用 0.5 N NaOH 或 0.4 M 乙酸盐缓冲液作为洗脱液可确保根据 Mw 进行 CMC 分离。结果表明，聚电解质 CMC 和中性聚合物葡聚糖的通用校准在所应用的条件下是有效的。 0.5 N NaOH 和 0.4 M 醋酸盐缓冲液中 CMC 的 Mark-Houwink 方程估计分别为 eta = 5.37x10(-4) M-w(0.73) 和 eta = 2.24x10(-4) M-w(0.83) dl g(-1)。已估计 CMC 的方程 log K = 1.64 - 4.00 alpha ml g(-1)。已经开发出一种通过 290 或 313 nm 的吸附测定 DO 的方法。 (C) 1998 Elsevier Science Ltd. 保留所有权利。
A method for determination of carboxymethyl cellulose (CMC) molecular weight (M-w) and chemical heterogeneity (degree of oxidation (DO)) using a bi-detector HPSEC (UV-detector online with refractometer) has been developed. It has been found that the use of 0.5 N NaOH or 0.4 M acetate buffer as the eluent ensures CMC separation according to Mw. It has been revealed that the universal calibration for the polyelectrolyte CMC and the neutral polymer dextran is valid under the conditions applied. The Mark-Houwink equations for CMC in 0.5 N NaOH and 0.4 M acetate buffer have been estimated to be [eta] = 5.37x10(-4) M-w(0.73) and [eta] = 2.24x10(-4) M-w(0.83) dl g(-1), respectively. The equation log K = 1.64 - 4.00 alpha ml g(-1) for CMC has been estimated. An approach for determining DO from adsorption at 290 or 313 nm has been developed. (C) 1998 Elsevier Science Ltd. All rights reserved.