Roles of the RAM signaling network in cell cycle progression in Saccharomyces cerevisiae
Roles of the RAM signaling network in cell cycle progression in Saccharomyces cerevisiae
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DOI：
10.1007/s00294-006-0069-y
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发表时间：
2006-06-01
期刊：
影响因子：
2.5
通讯作者：
Polymenis, Michael
中科院分区：
文献类型：
--
作者：
Bogomolnaya, Lydia M.;Pathak, Ritu;Polymenis, Michael
酿酒酵母Hym1p、Mob2p、Tao3p、Cbk1p、Sog2p和Kic1p蛋白被认为在RAM信号网络中一起起作用，该网络控制极化生长、细胞分离和细胞完整性。这些蛋白质是否也作为网络影响细胞增殖尚不清楚。在这里，我们检查了缺乏或过度表达RAM组件的细胞，并评估了每种情况下DNA复制起始的时间。我们的研究结果表明RAM蛋白的相反作用，其中只有Hym1p可以促进细胞周期从G1期到S期的过渡。我们还发现了添加剂生长缺陷的菌株缺乏几个成对的RAM蛋白的组合，可能会争论RAM组件在细胞增殖的整体控制的多个角色。最后，我们的研究结果表明，Hym1p需要Dcr2p磷酸酶来促进G1/S转换，但它不需要G1细胞周期蛋白Cln3p或RAS通路。综上所述，我们的研究结果表明RAM蛋白以一种以前没有预料到的非均匀方式对细胞增殖进行了复杂的调节。
The Saccharomyces cerevisiae Hym1p, Mob2p, Tao3p, Cbk1p, Sog2p and Kic1p proteins are thought to function together in the RAM signaling network, which controls polarized growth, cell separation and cell integrity. Whether these proteins also function as a network to affect cell proliferation is not clear. Here we examined cells lacking or over-expressing RAM components, and evaluated the timing of initiation of DNA replication in each case. Our results suggest opposing roles of RAM proteins, where only Hym1p can promote the transition from the G1 to S phase of the cell cycle. We also uncovered additive growth defects in strains lacking several pair-wise combinations of RAM proteins, possibly arguing for multiple roles of RAM components in the overall control of cell proliferation. Finally, our findings suggest that Hym1p requires the Dcr2p phosphatase to promote the G1/S transition, but it does not require the G1 cyclin Cln3p or the RAS pathway. Taken together, our results point to a complex regulation of cell proliferation by RAM proteins, in a non-uniform manner that was not previously anticipated.