Heterogeneity of oxygen delivery in normoxic and hypoxic states: a fluorometer study.
Heterogeneity of oxygen delivery in normoxic and hypoxic states: a fluorometer study.
复制标题
常氧和缺氧状态下氧气输送的异质性：荧光计研究。
作者：
B. Chance;C. Barlow;Y. Nakase;H. Takeda;A. Mayevsky;R. Fischetti;N. Graham;J. Sorge
飞点荧光计提供了在线实时直方图显示向灌注和原位器官输送氧气的异质性，该荧光计为线粒体空间的氧化黄素蛋白或线粒体和胞质空间的还原吡啶核苷酸提供激发。以 10(4) 至 10(5) 个数据点/秒的速度采集两种荧光染料的发射，并以每秒 1--10 次的速度显示荧光强度与该强度出现次数的直方图。直方图显示了具有冠状动脉闭塞模型的灌注心脏、灌注和原位大鼠肝脏以及中风的大鼠和沙鼠模型的氧化还原状态的强度和异质性程度的变化。输送到细胞内空间的氧气的百分比变化可以从直方图下的面积计算出来。
An on-line, real-time histogram display of heterogeneity of oxygen delivery to perfused and in situ organs is afforded by a flying-spot fluorometer that provides excitation for either oxidized flavoprotein of the mitochondrial space or reduced pyridine nucleotide of mitochondrial and cytosolic spaces. Emission from the two fluorochromes is acquired at 10(4) to 10(5) data points/s and histograms of the fluorescence intensity versus the number of occurrences of that intensity are displayed at 1--10 times per second. The histograms show alterations of the intensity and of the degree of heterogeneity of the redox states of perfused heart with model coronary occlusion, of perfused and in situ rat liver, and of rat and gerbil models of stroke. The percentage change of oxygen delivery to the intracellular space can be calculated from the areas under the histogram.