Increased content of type III collagen at the rupture site of human Achilles tendon
Increased content of type III collagen at the rupture site of human Achilles tendon
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DOI：
10.1016/s0736-0266(02)00064-5
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发表时间：
2002-11-01
影响因子：
2.8
通讯作者：
Risteli, J
中科院分区：
文献类型：
--
作者：
Eriksen, HA;Pajala, A;Risteli, J
我们比较了I型和III型胶原的数量和交联端肽在断裂部位和其他两个网站的同一肌腱。收集了十名跟腱完全断裂者和六具健康尸体的肌腱样本。通过提取可溶性前肽PINP、PICP和PIIINP来评估新合成的I型和III型前胶原。不溶性基质通过热变性和胰蛋白酶消化溶解。羟脯氨酸、I型胶原（ICTP和SP 4）和III型胶原（IIINTP）的交联端肽结构以及III型胶原的降解产物（胰蛋白酶PIIINP）在破裂部位与对照部位（增加5倍和12倍）或尸体（增加5倍）相比显著增加。未观察到新合成的I型和III型前胶原的量的变化。ICTP含量随年龄增长而下降，SP 4/ICTP比值沿着升高，提示I型胶原羧基端肽交联类型发生了结构变化，III型胶原在破裂部位的聚集可能与微创伤及随后的愈合过程有关。III型胶原含量的增加可导致胶原纤维变细，抗张强度降低，最终可能导致肌腱完全断裂。羧基末端肽中交联性质的年龄相关变化可能有助于这种弱化。(C)2002骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
We compared the type I and III collagen amounts and cross-linked telopeptides at the rupture site and two other sites of the same tendon. Tendon samples of ten individuals with total Achilles tendon rupture and six healthy cadavers were collected. The newly synthesized type I and III procollagens were assessed by extracting the soluble propeptides PINP, PICP and PIIINP. The insoluble matrix was solubilized by heat denaturation and trypsin digestion. Hydroxyproline, the cross-linked telopeptide structures of type I (ICTP and SP 4) and III collagens (IIINTP) and the degradation product of type III collagen (tryptic PIIINP) were measured from the digests.The type III collagen content was significantly increased at the rupture site when compared to control sites (5- and 12-fold increased) or cadavers (5-fold increased). No changes in the amounts of newly synthesized type I and III procollagens were observed. The ICTP content decreased and the SP 4/ICTP ratio increased along with ageing, suggesting a structural change in the type of cross-link in the carboxyterminal telopeptide of type I collagen.Type III collagen has accumulated at the rupture site probably due to microtraumas and the subsequent healing process. The increased content of type III collagen can cause thinner collagen fibers, decrease the tensile strength and may finally result in total rupture of the tendon. The age-related change in the nature of the cross-link in the carboxyterminal telopeptide may contribute to this weakening. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.