Hybrid Frequencies Confirm Limit to Coinfection in the RNA Bacteriophage φ6
Hybrid Frequencies Confirm Limit to Coinfection in the RNA Bacteriophage φ6
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DOI：
10.1128/jvi.73.3.2420-2424.1999
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发表时间：
1999-03
影响因子：
5.4
通讯作者：
P. Turner;C. Burch;K. Hanley;L. Chao
中科院分区：
文献类型：
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作者：
P. Turner;C. Burch;K. Hanley;L. Chao
摘要同一宿主细胞被多种病毒共感染可能导致对有限细胞资源的竞争加剧，从而降低单个病毒的适合度。选择应该有利于能够限制或防止合并感染的病毒，许多病毒已经进化出这样做的机制也就不足为奇了。在这里，我们探索联合感染是否仅限于感染菜豆假单胞菌的φ6噬菌体。我们通过比较两个标记噬菌体菌株产生的杂交频率与基于不同混合感染限制的数学模型预测的杂交频率，估计了φ6中混合感染的限制。我们的结果为估计联合感染的限度提供了另一种方法，并证实了先前每个宿主细胞两到三个噬菌体之间的估计。此外，我们的数据显示，在低噬菌体密度下的重叠感染率可能会超过随机泊松抽样的预期。我们讨论了噬菌体φ6是否进化出了一个最佳极限，以平衡与多重感染相关的代价高昂和有益的健身影响。
ABSTRACT Coinfection of the same host cell by multiple viruses may lead to increased competition for limited cellular resources, thus reducing the fitness of an individual virus. Selection should favor viruses that can limit or prevent coinfection, and it is not surprising that many viruses have evolved mechanisms to do so. Here we explore whether coinfection is limited in the RNA bacteriophage φ6 that infectsPseudomonas phaseolicola. We estimated the limit to coinfection in φ6 by comparing the frequency of hybrids produced by two marked phage strains to that predicted by a mathematical model based on differing limits to coinfection. Our results provide an alternative method for estimating the limit to coinfection and confirm a previous estimate between two to three phages per host cell. In addition, our data reveal that the rate of coinfection at low phage densities may exceed that expected through random Poisson sampling. We discuss whether phage φ6 has evolved an optimal limit that balances the costly and beneficial fitness effects associated with multiple infections.