Dax-1 as one of the target genes of Ad4BP/SF-1
Dax-1 as one of the target genes of Ad4BP/SF-1
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DOI：
10.1210/me.13.8.1267
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发表时间：
1999-08-01
影响因子：
--
通讯作者：
Morohashi, K
中科院分区：
文献类型：
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作者：
Kawabe, K;Shikayama, T;Morohashi, K
DAX-1（也称为 AHC）基因编码核激素受体超家族的一个不寻常成员。 DAX-1 在性腺和肾上腺分化过程中发挥着关键作用，因为人类 DAX-1 基因突变会导致与低促性腺激素性性腺功能减退相关的 X 连锁先天性肾上腺发育不全。在最近的研究中，据报道，DAX-1 充当 Ad4BP/SF-1 的转录抑制因子，而 Ad4BP/SF-1 是性腺和肾上腺分化中的关键转录因子。关于 Ad4BP/SF-1 在 DAX-1 基因转录调控中的作用，先前报道的结果不一致。我们研究了小鼠 Dax-1（也称为 Ahch）基因的上游区域，并通过瞬时转染和电泳迁移率变动测定鉴定了一个新的 Ad4/SF-1 位点。此外，使用 Dax-1 特异性抗体进行的免疫组织化学分析表明，类固醇生成组织、垂体和下丘脑中存在免疫反应细胞。尽管 Dax-1 和 Ad4BP/SF-1 的分布非常相似，但它们并不完全相同。在编码 Ad4BP/SF-1 的 Ftz-f1 基因敲除小鼠中，Dax-1 的表达显着受损。综上所述，我们的研究结果表明 Ad4BP/SF-1 控制 Dax-1 基因的转录。
The DAX-1 (also known as AHC) gene encodes an unusual member of the nuclear hormone receptor superfamily. DAX-1 plays a critical role during gonadal and adrenal differentiation since mutations of the human DAX-1 gene cause X-linked adrenal hypoplasia congenita associated with hypogonadotropic hypogonadism. In recent studies, DAX-1 was reported to function as a transcriptional suppressor of Ad4BP/SF-1, a critical transcription factor in gonadal and adrenal differentiation. With respect to implication of Ad4BP/SF-1 in the transcriptional regulation of the DAX-1 gene, inconsistent findings have been previously reported. We investigated the upstream region of the mouse Dax-1 (also known as Ahch) gene and identified a novel Ad4/SF-1 site by transient transfection and electrophoretic mobility shift assays. In addition, immunohistochemical analyses with a specific antibody to Dax-1 indicated the presence of immunoreactive cells in steroidogenic tissues, pituitary gland, and hypothalamus. Although the distributions of Dax-1 and Ad4BP/SF-1 were very similar, they were not completely identical. The expression of Dax-1 was significantly impaired in knock-out mice of the Ftz-f1 gene, which encodes Ad4BP/SF-1. Taken together, our findings indicate that Ad4BP/SF-1 controls the transcription of the Dax-1 gene.