Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer.
Iron imaging reveals tumor and metastasis macrophage hemosiderin deposits in breast cancer.
复制标题
DOI：
10.1371/journal.pone.0184765
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Koutcher JA
中科院分区：
文献类型：
--
作者：
Leftin A;Ben-Chetrit N;Klemm F;Joyce JA;Koutcher JA
铁沉积是正常和病理情况下巨噬细胞的代谢生物标志物，但铁在肿瘤和转移相关巨噬细胞中的存在尚不清楚。在这里，我们使用铁和巨噬细胞组织学以及体内MRI绘制并量化了转移性乳腺癌小鼠模型中富含含铁血黄素的巨噬细胞（HLM）沉积物。铁MRI检测到乳腺肿瘤，肺转移瘤，脑转移瘤以及已知含有HLM的肝脏和脾脏组织中的高铁像素簇。铁组织学显示这些区域包含聚集的巨噬细胞，其通过其共同的铁状态和与其他表型巨噬细胞标记物的组织内在关联来鉴定。进一步处理体内MRI和离体组织学图像以确定铁沉积物的频率和大小，并测量每个存款中HLM的数量以估计这些细胞的体内MRI灵敏度。含铁血黄素蓄积是一种巨噬细胞生物标志物，也是细胞MRI的内在造影剂来源，与巨噬细胞在全身铁代谢和转移性癌症中的先天功能相关。
Iron-deposition is a metabolic biomarker of macrophages in both normal and pathological situations, but the presence of iron in tumor and metastasis-associated macrophages is not known. Here we mapped and quantified hemosiderin-laden macrophage (HLM) deposits in murine models of metastatic breast cancer using iron and macrophage histology, and in vivo MRI. Iron MRI detected high-iron pixel clusters in mammary tumors, lung metastasis, and brain metastasis as well as liver and spleen tissue known to contain the HLMs. Iron histology showed these regions to contain clustered macrophages identified by their common iron status and tissue-intrinsic association with other phenotypic macrophage markers. The in vivo MRI and ex vivo histological images were further processed to determine the frequencies and sizes of the iron deposits, and measure the number of HLMs in each deposit to estimate the in vivo MRI sensitivity for these cells. Hemosiderin accumulation is a macrophage biomarker and intrinsic contrast source for cellular MRI associated with the innate function of macrophages in iron metabolism systemically, and in metastatic cancer.
登录
查看更多内容
影响因子：
3.3
作者：
GILLIS, P;KOENIG, SH
通讯作者：
KOENIG, SH
DOI：
10.1158/1078-0432.ccr-10-3420
发表时间：
2011-09-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Daldrup-Link HE;Golovko D;Ruffell B;Denardo DG;Castaneda R;Ansari C;Rao J;Tikhomirov GA;Wendland MF;Corot C;Coussens LM
通讯作者：
Coussens LM
DOI：
10.1186/1532-429x-16-40
发表时间：
2014-06-10
期刊：
Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance
影响因子：
--
作者：
Garbowski MW;Carpenter JP;Smith G;Roughton M;Alam MH;He T;Pennell DJ;Porter JB
通讯作者：
Porter JB
影响因子：
11.2
作者：
Lin, Elaine Y.;Li, Jiu-Feng;Pollard, Jeffrey W.
通讯作者：
Pollard, Jeffrey W.
DOI：
10.1016/s0925-4439(99)00104-0
发表时间：
2000-02-21
影响因子：
6.2
作者：
Allen, PD;St Pierre, TG;Rao, KV
通讯作者：
Rao, KV