Sensory genes identification with head transcriptome of the migratory armyworm, Mythimna separata
Sensory genes identification with head transcriptome of the migratory armyworm, Mythimna separata
复制标题
粘虫头部转录组的感觉基因鉴定
DOI：
10.1038/srep46033
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发表时间：
2017-04-07
影响因子：
4.6
通讯作者：
Zhu, Fen
中科院分区：
文献类型：
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作者：
Liu, Zhenxing;Wang, Xiaoyun;Zhu, Fen
感觉系统在昆虫的一系列行为和生理事件中发挥着重要作用，包括独立且顺序发生的寄主着陆和定位、摄食、飞行、性反应、交配和产卵。迁徙昆虫粘虫粘虫（鳞翅目：夜蛾科）对全世界的粮食作物和经济作物具有破坏性。在这里，我们展示了头部转录组的高通量测序，并鉴定了对粘虫在世界范围内的成功至关重要的主要感觉基因的成员，包括8种视蛋白、22种化学感应蛋白、50种气味结合蛋白、60种气味受体、8种味觉受体、24种离子型受体和2种感觉神经元膜蛋白。值得注意的是，这种昆虫中存在 LW 视蛋白基因的重复。还鉴定了一些基因与功能验证的基因聚集在一起，例如 OR 和 IR 的共同受体、PBP、PR、CO2 GR、苦味 GR 和甜味 GR。转录组基因库为进一步研究阐明M. separata的生物学和控制的基本分子机制提供了基础。我们的研究展示了第一个对分离分枝杆菌成功和分布至关重要的感觉基因的综合目录，这些基因是害虫控制策略的潜在新目标。
Sensory system plays important roles in a wide array of insect's behavior and physiological events, including the host landing and locating, feeding, flying, sex responding, mating and oviposition which happen independently and in sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) of migratory insect is destructive for alimentarn crop and economic crop throughout the world. Here we present the high throughput sequencing of the head transcriptome and identify members of the major sensory genes which are crucial for armyworm's success worldwide, including 8 opsins, 22 chemosensory proteins, 50 odorant binding proteins, 60 odorant receptors, 8 gustatory receptors, 24 ionotropic receptors, and 2 sensory neuron membrane proteins. It is worth noting that a duplication of the LW opsin gene exists in this insect. Several genes were clustered with functionally validated genes, such as Co-receptors of OR and IR, PBPs, PRs, CO2 GRs, bitter GRs and sweet GRs, were also identified. The transcriptome gene library provided the basis for further studies that elucidate the fundamental molecular mechanism of biology and control in M. separata. Our research exhibits the first comprehensive catalogue of the sensory genes fundamental for success and distribution in M. separata, which are potential novel targets for pest control strategies.