Application of gene expression profiling to colon cell maturation, transformation and chemoprevention
Application of gene expression profiling to colon cell maturation, transformation and chemoprevention
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DOI：
10.1093/jn/133.7.2410s
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发表时间：
2003-07-01
影响因子：
4.2
通讯作者：
Mariadason, JM
中科院分区：
文献类型：
--
作者：
Augenlicht, LH;Velcich, A;Mariadason, JM
同时检测数千个基因的高通量基因表达谱分析方法是理解和分类细胞和组织表型的有力方法。这包括分析细胞成熟的正常途径及其在转化过程中的扰动，正常细胞和肿瘤细胞对生理和药理药物的敏感性和反应机制，以及营养因子对肿瘤风险和进展的调节。然而，这种方法产生的复杂数据给分析带来了困难。我们将描述我们在分析实验室中许多项目中生成的数据库时使用的一些方法。其中包括：k-ras突变在结肠细胞转化中的作用；p21（WAF1/Cip1）在肠道肿瘤形成和舒林酸应答中的作用；吸收型和杯状细胞系的发展；结肠细胞对化疗药物的敏感性利用转录成像新方法调节c-myc表达的机制；以及营养和遗传因素在调节肠道肿瘤形成中的相互作用。
Methods for high-throughput analysis of profiles of gene expression that assay thousands of genes simultaneously are powerful approaches for understanding and classifying cell and tissue phenotype. This includes analysis of normal pathways of cell maturation and their perturbation in transformation, the sensitivity and mechanism of response of normal and tumor cells to physiological and pharmacological agents, and modulation of tumor risk and progression by nutritional factors. However, the complex data generated by such approaches raise difficulties in analysis. We will describe some of the methods we have used in analyzing databases generated in a number of projects in our laboratories. These include: the role of k-ras mutations in colon cell transformation; the role of p21(WAF1/Cip1) in intestinal tumor formation and response to sulindac; the development of the absorptive and goblet cell lineages; sensitivity of colonic cells to chemotherapeutic agents; mechanisms that regulate c-myc expression utilizing novel methods of transcriptional imaging; and interaction of nutritional and genetic factors in modulation of intestinal tumor formation.