EXPERIMENTAL DIABETIC KETOACIDOSIS - SEQUENTIAL-CHANGES OF METABOLIC INTERMEDIATES IN BLOOD, LIVER, CEREBROSPINAL-FLUID AND BRAIN AFTER ACUTE INSULIN DEPRIVATION IN STREPTOZOTOCIN-DIABETIC RAT
EXPERIMENTAL DIABETIC KETOACIDOSIS - SEQUENTIAL-CHANGES OF METABOLIC INTERMEDIATES IN BLOOD, LIVER, CEREBROSPINAL-FLUID AND BRAIN AFTER ACUTE INSULIN DEPRIVATION IN STREPTOZOTOCIN-DIABETIC RAT
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DOI：
10.1042/bj1380107
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发表时间：
1974-01-01
影响因子：
4.1
通讯作者：
ALBERTI, KGM
中科院分区：
文献类型：
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作者：
BLACKSHEAR, PJ;ALBERTI, KGM
静脉注射链脲佐菌素（150 mg/kg）使雄性大鼠患糖尿病，用长效胰岛素治疗1周，然后使其发生酮症酸中毒。通过使用旨在避免使用麻醉和延长缺氧期的采样技术，在最后一次胰岛素注射后24小时间隔对血液、肝脏、脑脊液和脑中的代谢中间产物进行连续测量。在血液和肝脏中的测量表明，在最后一次胰岛素注射后24和48小时之间，肝糖原分解和糖原异生以及外周贮库脂肪分解迅速增加，而血液和肝脏酮体和甘油三酯浓度上升较慢。随着胰岛素剥夺时间的增加而发生的代谢模式的变化强调了在实验性糖尿病中与静态测量相比顺序测量的重要性。糖尿病酮症酸中毒脑代谢中间体的数据，并支持最近的证据表明，葡萄糖在酮症状态下的脑氧化代谢中起着不太重要的作用。
Male rats rendered diabetic by the intravenous injection of streptozotocin (150mg/kg) were treated with a long-acting insulin for 1 week, then allowed to develop ketoacidosis. By using sampling techniques designed to avoid the use of anaesthesia and extended anoxic periods, sequential measurements of metabolic intermediates were made in blood, liver, cerebrospinal fluid and brain at 24h intervals after the last insulin injection. Measurements in blood and liver suggested a rapid increase in hepatic glycogenolysis and gluconeogenesis and peripheral-depot lipolysis between 24 and 48h after the last insulin injection, whereas blood and liver ketone-body and triglyceride concentrations rose more slowly. The changing metabolic patterns occurring with increasing time of insulin deprivation stress the importance of sequential compared with static measurements in experimental diabetes. Data are presented for brain metabolic intermediates in diabetic ketoacidosis, and support recent evidence that glucose plays a less important role in brain oxidative metabolism in ketotic states.