IGF-I and bFGF improve dopamine neuron survival and behavioral outcome in parkinsonian rats receiving cultured human fetal tissue strands
IGF-I and bFGF improve dopamine neuron survival and behavioral outcome in parkinsonian rats receiving cultured human fetal tissue strands
复制标题
DOI：
10.1006/exnr.2000.7593
复制
发表时间：
2001-03-01
影响因子：
5.3
通讯作者：
Freed, CR
中科院分区：
文献类型：
--
作者：
Clarkson, ED;Zawada, WM;Freed, CR
为了促进多巴胺细胞在移植到免疫抑制的6-羟多巴胺损伤大鼠体内的人胎组织链中的存活，我们将组织在胰岛素样生长因子- 1 （IGF-I, 150 ng/ml）和碱性成纤维细胞生长因子（bFGF, 15 ng/ml）中体外预孵育2周。生长因子处理不影响高香草酸的体外生成率，但使移植后动物多巴胺神经元的总存活率从1240 +/- 250增加到2380 +/- 440 （P < 0.05）。与对照移植相比，生长因子处理组的动物甲基苯丙胺诱导的旋转（66%）明显减少（30%,P < 0.05）。我们得出结论，igf - 1和bFGF在体外预孵育人胎儿组织链可改善多巴胺细胞存活和移植的行为结果。(C) 2001学术出版社。
To promote dopamine cell survival in human fetal tissue strands transplanted into immunosuppressed 6-OHDA-lesioned rats, we have preincubated tissue in insulin-like growth factor-I (IGF-I, 150 ng/ml) and basic fibroblast growth factor (bFGF, 15 ng/ml) in vitro for 2 weeks. Growth factor treatment did not affect the rate of homovanillic acid production in vitro but increased overall dopamine neuron survival in animals after transplant from 1240 +/- 250 to 2380 +/- 440 neurons (P < 0.05). Animals in the growth factor-treated group had a significantly greater reduction in methamphetamine-induced rotation (66%) compared to control transplants (30%, P < 0.05). We conclude that in vitro preincubation of human fetal tissue strands with IGF-I and bFGF improves dopamine cell survival and the behavioral outcome of transplants. (C) 2001 Academic Press.