Adsorption of Bovine Serum Albumin and lysozyme on siliceous MCM-41
Adsorption of Bovine Serum Albumin and lysozyme on siliceous MCM-41
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DOI：
10.1016/j.micromeso.2004.11.026
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发表时间：
2005-05
影响因子：
5.2
通讯作者：
Amit Katiyar;L. Ji;P. Smirniotis;N. Pinto
中科院分区：
文献类型：
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作者：
Amit Katiyar;L. Ji;P. Smirniotis;N. Pinto
研究了MCM-41对牛血清白蛋白（BSA）和溶菌酶的吸附。特别是，对使用这种吸附剂作为蛋白质分离的色谱支持的重要特性已经进行了检查。这些因素包括外表面积、溶液条件（pH）和吸附速率的影响。对三种孔径的MCM-41进行了对比研究（24.8Å， 36Å， 53.5Å）。研究表明，外表面积的差异对吸附能力有很大影响。这意味着外表面的吸附可以被误解为MCM-41孔隙中的吸附。因此，高吸附能力不一定意味着高分子筛分能力。本文还报道了蛋白质摄取速率对溶液条件的依赖性。量热法表明，在一定的溶液条件下，牛血清白蛋白的吸附是两个连续能量事件的结合。这些假设是BSA吸附，然后是表面吸附蛋白质的重定向/重组。缓慢的第二步影响平衡的实现。
The adsorption of Bovine Serum Albumin (BSA) and lysozyme on MCM-41 was studied. In particular, characteristics important to the use of this adsorbent as a chromatographic support for protein separations have been examined. These include the effects of external surface area, solution conditions (pH), and rate of adsorption. Comparative studies were performed on MCM-41 of three pore sizes (24.8Å, 36Å, 53.5Å). It has been shown that differences in external surface area can strongly influence the adsorption capacity. This implies that adsorption on the external surface can be misinterpreted as adsorption in the pores of MCM-41. Thus, high adsorption capacities may not necessarily mean high molecular-sieving capacities. This paper also reports a dependence of protein uptake rates on solution conditions. Calorimetry suggests that under certain solution conditions the adsorption of BSA is a combination of two sequential energetic events. These are postulated to be BSA adsorption followed by reorientation/reconformation of the adsorbed protein on the surface. The slow second step affects the attainment of equilibrium.