Human interleukin 2 can promote the growth and differentiation of single hapten-specific B cells in the presence of specific antigen.
Human interleukin 2 can promote the growth and differentiation of single hapten-specific B cells in the presence of specific antigen.
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人白细胞介素2在特定抗原存在下可促进单个半抗原特异性B细胞的生长和分化。
DOI：
10.1073/pnas.81.24.7917
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发表时间：
1984
影响因子：
11.1
通讯作者：
Nossal,GJ
中科院分区：
文献类型：
--
作者：
Pike,BL;Raubitschek,A;Nossal,GJ
用半抗原-明胶亲和法从正常成年小鼠脾脏中制备了对半抗原荧光素（FLU）具有特异性反应的B淋巴细胞。将它们单独或在没有任何饲养细胞、填充细胞或辅助细胞的情况下少量放置在10微升微量培养物中。使用“T细胞非依赖性”抗原FLU-POL缀合的聚合鞭毛蛋白（FLU-POL）或选定批次的FLU-POL缀合的Ficoll，并且这些刺激分裂和分化仅在同时存在充当B细胞生长和分化因子（BGDF）的淋巴因子的情况下。结果发现，人白细胞介素2（IL-2），制备的重组DNA技术（r-IL-2），是一种有效的，虽然相当弱，BGDF在这个系统中。当不含IL-2的BGDF来源与抗原刺激物一起使用时，加入r-IL-2并不增加应答，从粗品淋巴因子混合物中去除IL-2也不降低BGDF活性。
B lymphocytes specifically reactive to the hapten fluorescein (FLU) were prepared from normal adult murine spleen by the hapten-gelatin affinity procedure. They were placed in 10 microliter of microcultures singly or in small numbers in the absence of any feeder, filler, or accessory cell. The "T cell-independent" antigen FLU-conjugated polymerized flagellin (FLU-POL) or a selected batch of FLU-conjugated Ficoll were used, and these stimulated division and differentiation only in the concomitant presence of lymphokines acting as B-cell growth and differentiation factors (BGDF). It was found that human interleukin 2 (IL-2), prepared by recombinant DNA technology (r-IL-2), was an effective, albeit rather weak, BGDF in this system. When an IL-2-free source of BGDF was used with the antigenic stimulus, addition of r-IL-2 did not augment the response, nor did removal of IL-2 from the crude lymphokine mixture diminish the BGDF activity.