Quantification of human plasma inorganic pyrophosphate
Quantification of human plasma inorganic pyrophosphate
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人血浆无机焦磷酸盐的定量
DOI：
10.1002/art.1780220813
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发表时间：
1979
影响因子：
--
通讯作者：
D. Mccarty
中科院分区：
文献类型：
--
作者：
L. Ryan;F. Kozin;D. Mccarty
血浆无机焦磷酸盐（PPi）水平在剧烈全身运动后可预见地升高（28%），并在休息30分钟后恢复到基线值。股静脉血中血浆PPi水平高于股动脉血（36%）。然而，前臂肌肉锻炼并没有导致肘前静脉血浆PPi水平的可检测升高。全身运动效应和动静脉差异的生理原因仍然未知。在基础条件下连续10天测量单个正常受试者的血浆PPi，其中这些和其他先前确定的生物变量（空腹和昼夜变化）得到控制，显示变异系数为12.7%。因此，与方法学误差相比，生物学变异性对观察到的血浆PPi变异的贡献较小（方法变异系数= 8%）。血浆PPi在该受试者中的惊人再现性表明，这种重要的代谢物处于相当严格的稳态控制之下。
Plasma inorganic pyrophosphate (PPi) levels rose predictably (28%) after vigorous systemic exercise and returned to baseline values after 30 minutes of rest. Plasma PPi levels were greater (36%) in femoral venous blood than in femoral arterial blood. Forearm muscular exercise, however, did not result in a detectable rise in plasma PPi levels in antecubital veins. The physiologic reasons for both the systemic exercise effect and the arteriovenous differences remain unknown. Measurement of plasma PPi for 10 consecutive days in a single normal subject under basal conditions, where these and other previously identified biologic variables (fasting and diurnal variation) were controlled, showed a coefficient of variation of 12.7%. Thus biologic variability contributed less to observed variation in plasma PPi than did methodologic errors (coefficient of variation of method = 8%). The striking reproducibility of plasma PPi in this subject suggests that this important metabolite is under rather tight homeostatic control.