Genetic background of enhanced radioresistance in an anhydrobiotic insect: transcriptional response to ionizing radiations and desiccation
Genetic background of enhanced radioresistance in an anhydrobiotic insect: transcriptional response to ionizing radiations and desiccation
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DOI：
10.1007/s00792-016-0888-9
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发表时间：
2016-11
期刊：
影响因子：
2.9
通讯作者：
A. Ryabova;Kyosuke Mukae;A. Cherkasov;R. Cornette;E. Shagimardanova;T. Sakashita;T. Okuda;T. Kikawada;O. Gusev
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文献类型：
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作者：
A. Ryabova;Kyosuke Mukae;A. Cherkasov;R. Cornette;E. Shagimardanova;T. Sakashita;T. Okuda;T. Kikawada;O. Gusev
据推测，对电离辐射的抵抗力以及对其他非生物胁迫的交叉抵抗力是脱水动物在进化基础上适应脱水胁迫的副作用。Vanderelican的幼虫能够承受长时间的干燥以及高剂量的电离辐射暴露。为了进一步了解这两种类型的应激暴露的交叉耐受机制，我们使用微阵列技术分析了在不同干燥阶段收集的摇蚊幼虫的全基因组mRNA表达模式，并暴露于两种类型的电离辐射-70戈伊的高线性能量转移（LET）离子（4 He）和相同剂量的低LET辐射（γ射线）。在表达谱中，观察到对干燥胁迫的广泛转录响应，远远超过了对辐射暴露的上调转录物的量。在干旱和电离辐射胁迫下，发现了大量的重叠转录本，其中包括抗氧化剂、胚胎发育后期丰富（莱亚）蛋白和热休克蛋白等脱水相关基因。最高表达的组是蛋白-L-异天冬氨酸/D-天冬氨酸O-甲基转移酶（PIMT），而对应于莱亚蛋白的探针是最具代表性的。进行功能分析显示，强烈丰富的基因本体论术语与蛋白质甲基化。此外，还检测到DNA修复的活跃过程。我们假设，交叉耐受性的睡眠摇蚊干燥和辐射暴露来自一个复杂的机制，适应脱水。
It is assumed that resistance to ionizing radiation, as well as cross-resistance to other abiotic stresses, is a side effect of the evolutionary-based adaptation of anhydrobiotic animals to dehydration stress. Larvae ofPolypedilum vanderplankican withstand prolonged desiccation as well as high doses of ionizing radiation exposure. For a further understanding of the mechanisms of cross-tolerance to both types of stress exposure, we profiled genome-wide mRNA expression patterns using microarray techniques on the chironomid larvae collected at different stages of desiccation and after exposure to two types of ionizing radiation—70 Gy of high-linear energy transfer (LET) ions (4He) and the same dose of low-LET radiation (gamma rays). In expression profiles, a wide transcriptional response to desiccation stress that much exceeded the amount of up-regulated transcripts to irradiation exposure was observed. An extensive group of coincidently up-regulated overlapped transcripts in response to desiccation and ionizing radiation was found. Among this, overlapped set of transcripts was indicated anhydrobiosis-related genes: antioxidants, late embryogenesis abundant (LEA) proteins, and heat-shock proteins. The most overexpressed group was that of protein-L-isoaspartate/D-aspartateO-methyltransferase (PIMT), while probes, corresponding to LEA proteins, were the most represented. Performed functional analysis showed strongly enriched gene ontology terms associated with protein methylation. In addition, active processes of DNA repair were detected. We assume that the cross-tolerance of the sleeping chironomid to both desiccation and irradiation exposure comes from a complex mechanism of adaptation to anhydrobiosis.