Revisiting the optimal c value for isothermal titration calorimetry
Revisiting the optimal c value for isothermal titration calorimetry
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DOI：
10.1016/j.ab.2011.07.027
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发表时间：
2011-11-15
影响因子：
2.9
通讯作者：
Keller, Sandro
中科院分区：
文献类型：
--
作者：
Broecker, Jana;Vargas, Carolyn;Keller, Sandro
从等温滴定量热法获得的解离常数K（D）的精度取决于相互作用物质的浓度等因素。对于可靠的K（D）测定，所谓的c值（分析物浓度与K（D）的比率）应落在1至1000的范围内。根据模拟的无噪声数据，Biswas和Tsodikov [5]最近提出了5至20的最佳c值。相比之下，我们发现在c > 40时，通过包含最先进的微量热计典型的噪声水平的模拟来确定K（D）置信区间的最佳值。(C)2011 Elsevier Inc. All rights reserved.
The precision with which the dissociation constant, K(D), can be obtained from isothermal titration calorimetry depends on, among other factors, the concentrations of the interacting species. The so-called c-value the ratio of analyte concentration to K(D)-should fall in the range of 1 to 1000 for reliable K(D) determination. On the basis of simulated, noise-free data, Biswas and Tsodikov [5] recently suggested an optimal c value of 5 to 20. By contrast, we find an optimum at c > 40 on determining the K(D) confidence intervals through simulations containing noise levels typical of state-of-the-art microcalorimeters. (C) 2011 Elsevier Inc. All rights reserved.