IL-4 release by human leukemic and activated normal basophils.
IL-4 release by human leukemic and activated normal basophils.
复制标题
人类白血病和激活的正常嗜碱性粒细胞释放 IL-4。
DOI：
10.4049/jimmunol.151.3.1441
复制
发表时间：
1993
影响因子：
4.4
通讯作者：
P. Debré
中科院分区：
文献类型：
--
作者：
M. Arock;H. Merle;B. Dugas;F. Ouaaz;L. Le Goff;I. Vouldoukis;J. Mencia;C. Schmitt;V. Leblond;P. Debré
最近，作者已经解决了人类嗜碱性粒细胞产生IL-4的能力。我们在这里报告检测显着的血清IL-4水平的情况下，急性转化的慢性粒细胞白血病与主要嗜碱性细胞群体。从患者的PBMC中分离白血病嗜碱性粒细胞，并测定它们的IL-4-mRNA表达和它们在体外分泌这种细胞因子的能力。白血病嗜碱性细胞（> 90%甲苯胺蓝阳性）而非其他PBMC表达IL-4-mRNA，含有IL-4蛋白，并分泌这种细胞因子。这些细胞具有自发的IL-4分泌能力，而不需要外源性激活剂。同时，IL-4的释放显着增加白血病细胞激活后，通过Fc β RI连接或Ca 2+离子载体。IL-4及其mRNA也检测到白血病嗜碱性粒细胞从其他三个慢性粒细胞白血病患者中度嗜碱性粒细胞（13，14，和23%的PBMC嗜碱性粒细胞）。为了在正常人细胞中证实这些数据，我们开发了一种从人骨髓细胞培养中获得大量纯化嗜碱性粒细胞的方法。与白血病嗜碱性粒细胞相反，正常细胞需要通过Fc ε RI连接或通过Ca 2+离子载体体外活化以表达和分泌IL-4。白血病和正常嗜碱性粒细胞分泌组胺体外激活后，但类胰蛋白酶呈阴性。因此，这些数据证明了人嗜碱性粒细胞在体内和体外产生IL-4的能力。
Recently, authors have addressed the ability of human basophils to produce IL-4. We report here the detection of significant serum IL-4 levels in a case of acute transformation of chronic myelogenous leukemia with a predominant basophilic cell population. Leukemic basophils were isolated from patients' PBMC and assayed for their IL-4-mRNA expression and their ability to secrete this cytokine in vitro. Leukemic basophilic cells (> 90% toluidine blue positive) but not other PBMC expressed IL-4-mRNA, contained IL-4 protein, and secreted this cytokine. These cells had a spontaneous IL-4 secretion ability, without a need for an exogenous activator. Meanwhile, IL-4 release was significantly increased following leukemic cell activation through Fc epsilon RI-ligation or by Ca2+ ionophore. IL-4 and its mRNA were also detected in leukemic basophils from three other chronic myelogenous leukemia patients with moderate basophilia (13, 14, and 23% basophils in PBMC). To confirm these data in normal human cells, we have developed a method to obtain large numbers of purified basophils from human bone marrow cell cultures. In contrast to leukemic basophils, normal cells required in vitro activation through Fc epsilon RI ligation or by Ca2+ ionophore to express and secrete IL-4. Leukemic and normal basophils secreted histamine following in vitro activation, but were negative for tryptase. These data thus demonstrate the in vivo and in vitro ability of human basophils to produce IL-4.