Overexpression of δ-catenin is associated with a malignant phenotype and poor prognosis in colorectal cancer.
Overexpression of δ-catenin is associated with a malignant phenotype and poor prognosis in colorectal cancer.
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δ-连环蛋白的过度表达与结直肠癌的恶性表型和不良预后相关
DOI：
10.3892/mmr.2015.3918
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发表时间：
2015-09
影响因子：
3.4
通讯作者：
Dai CL
中科院分区：
文献类型：
--
作者：
Zhang H;Dai SD;Liu SL;Zhang FY;Dai CL
关于δ-连环蛋白（连环蛋白家族的成员）在结直肠癌（CRC）中的表达或临床意义知之甚少。本研究采用免疫组织化学方法检测了110例大肠癌组织中δ-catenin的表达，其中70例有完整随访记录，40例有配对淋巴结转移。同时采用逆转录定量聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blot）检测30例大肠癌和30例正常大肠组织中δ-catenin mRNA和蛋白的表达。δ-Catenin在正常肠上皮细胞胞浆中弱表达或不表达，而在结直肠癌细胞胞浆中呈阳性表达。结直肠癌组织中δ-catenin的阳性表达率（68.18%; 75/110）明显高于正常大肠组织（36.7%; 11/30; P<0.001）。结直肠癌组织中δ-catenin mRNA和蛋白的表达均显著高于癌旁正常组织（P均<0.05）。Ⅲ-IV期结直肠癌中δ-catenin的表达高于Ⅰ-II期结直肠癌，有淋巴结转移的患者肿瘤中δ-catenin的表达高于无淋巴结转移的患者。Kaplan-Meier生存曲线显示δ-catenin阳性表达患者的生存时间短于δ-catenin阴性表达患者（P=0.005）。考克斯多因素分析显示，肿瘤分期、淋巴结转移（P=0.02）和δ-catenin阳性表达（P=0.033）是影响大肠癌预后的独立因素。因此，本研究表明δ-catenin可能是一个合适的独立的预后因素的CRC。
Little is known regarding the expression or clinical significance of δ-catenin, a member of the catenin family, in colorectal cancer (CRC). The present study examined the expression of δ-catenin using immunohistochemistry in 110 cases of CRC, including 70 cases with complete follow-up records and 40 cases with paired lymph node metastases. In addition, δ-catenin mRNA and protein expression were compared in 30 pairs of matched CRC and normal colorectal tissues by reverse transcription quantitative polymerase chain reaction and western blot analysis. δ-Catenin was weakly expressed or absent in the cytoplasm of normal intestinal epithelial cells, whereas positive δ-catenin expression local-ized to the cytoplasm was observed in CRC cells. The rate of positive δ-catenin expression in CRC (68.18%; 75/110) was significantly higher than that in normal colorectal tissues (36.7%; 11/30; P<0.001). In addition, δ-catenin mRNA and protein expression were significantly increased in CRC tissues compared to those in their matched normal tissues (all P<0.05). The expression of δ-catenin in stage III–IV CRC was higher than that in stage I–II CRC, and the expression of δ-catenin in the tumors of patients with lymph node metastases was higher than that in patients without lymph node metastases. Kaplan-Meier survival curves demonstrated that the survival time of patients with positive δ-catenin expression was shorter than that of patients with negative δ-catenin expression (P=0.005). Furthermore, Cox multivariate analysis indicated that the tumor, nodes and metastasis stage (P=0.02) and positive δ-catenin expression (P=0.033) were independent prognostic factors in CRC. The present study therefore indicated that δ-catenin may be a suitable independent prognostic factor for CRC.