The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion
The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion
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DOI：
10.1128/jb.182.2.498-503.2000
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发表时间：
2000-01-01
影响因子：
3.2
通讯作者：
Valvano, MA
中科院分区：
文献类型：
--
作者：
Amer, AO;Valvano, MA
大肠杆菌WecA（Rfe）的翻译起始的正确位点，推测参与催化N-乙酰葡糖胺1-磷酸转移到十一异戊二烯磷酸，确定通过使用其FLAG标记的衍生物。含有三个预测的跨膜螺旋的N-末端区域被认为是必要的功能，但不是膜定位的这种蛋白质。
The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.