Characterization of secretory acid phosphatase gene of lupin roots and the transformation of the gene into tobacco plants
Characterization of secretory acid phosphatase gene of lupin roots and the transformation of the gene into tobacco plants
复制标题
羽扇豆根部分泌型酸性磷酸酶基因的鉴定及该基因向烟草植物中的转化
DOI：
10.1007/0-306-47624-x_16
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Tadano
中科院分区：
文献类型：
--
作者：
J. Wasaki;Hiraki Dateki;T. Shinano;M. Osaki;T. Tadano
羽扇豆（Lupinus albusL.）植物在缺磷条件下分泌大量的分泌型酸性磷酸酶（S-AP酶）。如果将该酶的功能转移到分泌S-AP酶能力较低的其他作物中，可以预期提高根际有机磷化合物的磷利用率。在这项研究中，我们分离和表征编码羽扇豆S-AP酶的基因。用编码羽扇豆S-AP酶的cDNA转移烟草植物。从转化体的根中分泌来源于导入的cDNA的S-AP酶。
Lupin (Lupinus albusL.) plants exudate a large amount of secretory acid phosphatase (S-APase) under phosphorus-deficient condition. If the function of this enzyme is transferred to other crop plants which had a lower secreting ability for S-APase, one may expect an improvement of phosphorus utilization from organic phosphorus compounds in the rhizosphere. In this study, we isolated and characterized the gene encoding lupin S-APase. Tobacco plants were transferred with the cDNA encoding lupin S-APase. S-APase derived from the introduced cDNA was secreted from roots of transformants.