A protein interaction map of Drosophila melanogaster
A protein interaction map of Drosophila melanogaster
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DOI：
10.1126/science.1090289
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发表时间：
2003-12-05
期刊：
影响因子：
56.9
通讯作者：
Rothberg, JM
中科院分区：
文献类型：
--
作者：
Giot, L;Bader, JS;Rothberg, JM
黑腹果蝇是人类生物学许多方面的成熟模型系统。在这里，我们提出了一个双杂交为基础的蛋白质相互作用的苍蝇蛋白质组图。总共分离出10，623个预测的转录物，并针对标准和归一化的互补DNA文库进行筛选，以产生7048个蛋白质和20，405个相互作用的草图。提出了一种计算双杂交相互作用置信度的方法。将该草图更新为4679种蛋白质和4780种相互作用的更高置信度的图谱。网络的统计建模显示了两个层次的组织：一个是短程组织，大概对应于多蛋白质复合物，另一个是更全局的组织，大概对应于复合物间的连接。该网络概括了已知的途径，扩展了途径，并发现了以前未知的途径成分。这张地图是包括人类在内的多细胞生物系统生物学建模的起点。
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to re. ne this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.