Performance Evaluation of a High-field Orbitrap Mass Analyzer
Performance Evaluation of a High-field Orbitrap Mass Analyzer
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DOI：
10.1016/j.jasms.2009.01.005
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发表时间：
2009-08-01
影响因子：
3.2
通讯作者：
Lange, Oliver
中科院分区：
文献类型：
--
作者：
Makarov, Alexander;Denisov, Eduard;Lange, Oliver
介绍了Orbitrap(TM)质量分析仪的一种新设计。通过减小内外轨道电极之间的间隙，在固定的采集时间内获得更高的离子振荡频率，从而获得更高的分辨率，从而在给定的电压下提供更高的场强。实验结果证实，最大半高宽分辨率在m/z 524时超过350,000，在m/z 195时超过600,000，蛋白质的同位素分辨率超过40 kDa，单次激发动态范围为25,000。研究还发现，新设计中的质量位移与分析器内部的空间电荷几乎没有关系。这一性能是通过使用更高的电压并通过仔细平衡结构公差和操作参数来实现的，与标准Orbitrap分析仪相比，这些公差和操作参数似乎在较窄的定时范围内变化。(J am Soc MS Spectrm 2009，20,1391-1396)(C)2009，Elsevier Inc.代表美国质谱学学会出版
A new design of the Orbitrap (TM) mass analyzer is presented. Higher frequencies of ion oscillations and hence higher resolving power over fixed acquisition time are achieved by decreasing the gap between the inner and outer Orbitrap electrodes, thus providing higher field strength for a given voltage. Experimental results confirm maximum FWHM resolving power in excess of 350,000 at m/z 524 and 600,000 at m/ z 195, isotopic resolution of proteins above 40 kDa, and a single-shot dynamic range of 25,000. It was also found that mass shifts in the new design depend very little on space charge inside the analyzer. This performance was achieved using higher voltages and by careful balancing of construction tolerances and operation parameters, which appeared to vary in narrower ranges of timing than for a standard Orbitrap analyzer. (J Am Soc Mass Spectrom 2009, 20, 1391-1396) (C) 2009 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry