Increased Chondrocyte Death after Steroid and Local Anesthetic Combination
Increased Chondrocyte Death after Steroid and Local Anesthetic Combination
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DOI：
10.1007/s11999-010-1443-0
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发表时间：
2010-11-01
影响因子：
4.2
通讯作者：
Bardos, Tamas
中科院分区：
文献类型：
--
作者：
Farkas, Boglarka;Kvell, Krisztian;Bardos, Tamas
透明关节软骨的修复和再生能力有限。关节内注射糖皮质激素和局麻药在骨关节炎的治疗中起着重要作用。据报道糖皮质激素和麻醉剂增强软骨细胞的凋亡，问题/目的探讨糖皮质激素和局麻药联合应用对软骨细胞凋亡是否有协同作用结果糖皮质激素和局麻药以不同的速率诱导软骨细胞凋亡。当联合使用时，在体外软骨细胞培养物和骨软骨细胞离体标本中死亡软骨细胞的百分比增加。结论我们观察到在类固醇和局部麻醉剂同时暴露后软骨细胞活力的时间依赖性降低。临床相关性糖皮质激素和局部麻醉剂的组合对关节软骨细胞有不良影响，并且它提出了关于在治疗骨关节炎中是否应该使用伴随给药的问题。
Background Hyaline articular cartilage has limited repair and regeneration capacity. Intraarticular administration of glucocorticoid and local anesthetic injections play an important role in the therapy of osteoarthritis. Glucocorticoids and anesthetics reportedly enhance apoptosis in chondrocytes, but effects of the combined use of glucocorticoids and local anesthetics are unknown.Questions/purposes We asked whether glucocorticoid and local anesthetic agents combined had any synergistic effects on chondrocyte apoptosis.Methods Cell viability and apoptosis/necrosis assessment of human articular chondrocytes were performed in vitro (chondrocyte cell cultures) and ex vivo (osteochondral specimens) using flow cytometry and TUNEL analysis, respectively.Results Glucocorticoids and local anesthetics induce apoptosis in chondrocytes at various rates. When used in combination, the percentage of dead chondrocytes was increased in in vitro chondrocyte cell cultures and osteochondral ex vivo specimens.Conclusions We observed a time-dependent decrease in chondrocyte viability after concurrent steroid and local anesthetic exposure.Clinical Relevance The combination of glucocorticoids and local anesthetics has an adverse effect on articular chondrocytes, and it raises a question regarding whether concomitant administration should be used in treating osteoarthritis.