Immunohistochemical localization of kininogen in rat spinal cord and brain.
Immunohistochemical localization of kininogen in rat spinal cord and brain.
复制标题
大鼠脊髓和大脑中激肽原的免疫组织化学定位。
DOI：
10.1006/exnr.1999.7165
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hogan,EL
中科院分区：
文献类型：
--
作者：
Li,Z;Tyor,WR;Xu,J;Chao,J;Hogan,EL
激肽原定位已确定在大鼠脊髓和脑中使用激肽定向的单克隆抗体的免疫细胞化学。在脊髓中，有免疫染色的神经元和纤维在板层I，II，VII和IX，强烈染色的纤维在背角的浅层，免疫反应性胶质细胞和内皮细胞。背根神经节内小神经元、卫星细胞和雪旺细胞免疫组化染色明显。脑干内孤束和孤束核、三叉神经脊束和三叉神经脊束核、导水管周围灰质、前庭神经核、耳蜗核、斜方体、内侧膝状体核和红核内均有免疫反应阳性神经元和纤维。免疫染色的神经元和纤维也被发现在小脑（齿状核），大脑皮层（第III和V层），海马（锥体细胞层），和胼胝体。胶质细胞和内皮细胞在所有脑区染色。激肽原在神经元及其过程中，以及在神经胶质细胞和内皮细胞中的广泛位置表明，在各种神经活动和反应中，不止一种功能作用，包括作为介质，钙蛋白酶抑制剂和激肽前体的功能。
Kininogen localization has been determined by immunocytochemistry in rat spinal cord and brain using a kinin-directed kininogen monoclonal antibody. In the spinal cord, there were immunostained neurons and fibers in laminae I, II, VII, and IX, intensely stained fibers in the superficial layers of the dorsal horn, and immunoreactive glial and endothelial cells. Small neurons, satellite cells, and Schwann cells immunostained distinctly in the dorsal root ganglion. In the brain stem, there were immunoreactive neurons and fibers in the tractus solitarius and nucleus, trigeminal spinal tract and nuclei, periaqueductal gray matter, vestibular nuclei, cochlear nuclei, trapezoid body, medial geniculate nucleus, and red nucleus. Immunostained neurons and fibers were also found in cerebellum (dentate nucleus), cerebral cortex (layers III and V), hippocampus (pyramidal cell layer), and corpus callosum. Glia and endothelial cells stained in all brain regions. The widespread location of kininogen in neurons and their processes, as well as in glial and endothelial cells, indicates more than one functional role, including those proposed as a mediator, a calpain inhibitor, and a kinin precursor, in a variety of neural activities and responses.